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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16937-50 | 50 µg (1 mg/ml) | $460 |
1 of 2
2 of 2
PathPlus™ Monoclonal Mouse anti‑Human CR1 / CD35 Antibody (clone E11, Azide‑free,BSA‑free, IHC, IF) LS‑B16937
PathPlus™ Monoclonal Mouse anti‑Human CR1 / CD35 Antibody (clone E11, Azide‑free,BSA‑free, IHC, IF) LS‑B16937
Note: This antibody replaces LS-C389528
Antibody:
CR1 / CD35 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (E11) Antibody
Application:
IHC-P, IF, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free
Other formats:
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CR1 / CD35 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (E11) Antibody
Application:
IHC-P, IF, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free
Other formats:
Specifications
Description
CD35 (also known as complement receptor type 1, CR1 or complement C3b/C4b receptor) is a membrane-bound protein member of the regulators of complement activation family. It mediates the immune adherence phenomenon; cellular binding to particles/immune complexes with activated complement. It also mediates inhibitory signals in B lymphocytes. CD35 is expressed at the cell surface in erythrocytes, dendritic cells, leukocytes, and podocytes. Polymorphisms and mutations of the CD35 gene have been reported in systemic lupus erythematosus, mesangiocapillary glomerulonephritis, gallbladder carcinoma, and sarcoidosis. Staining is used to identify dendritic cells and follicular dendritic cell tumors.
References: Immunol Res. 1987;6(3):192-209. PMID: 2960763; Proc R Soc Med. 1956;49(1):55-58. PMID: 13289834; J Immunol. 2002;168(6):2782-2788. PMID: 11884446; Mol Immunol. 2009;46(14)2767-2773. doi: 10.1016/j.molimm.2009.05.181; Hum Genet. 2005;118(2):225-234. doi: 10.1007/s00439-005-0044-6; Nephron. 1986;43(4):307. doi: 10.1159/000183861; Am J Surg Pathol. 1996;20(8):944-955. PMID: 8712294.
Target
Human CR1 / CD35
Synonyms
CR1 | C3b/C4b receptor | C3-binding protein | CD35 | C3BR | Knops blood group antigen | C4BR | CD35 antigen | Complement receptor type 1 | KN
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Clone
E11
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Azide-free, BSA-free.
Also available Unmodified or Ready-to-Use.
Immunogen
Intact human monocytes were used as the immunogen for this CD35 antibody.
Specificity
Human CR1 / CD35
Applications
- IHC - Paraffin (10 µg/ml)
- Immunofluorescence (1 - 2 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This antibody recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1), or CD35. The antibody is specific for a site on CD35 that is distal from the C3b-binding site, so that it is unable to block CD35 activity. The CD35 antibody is highly specific and shows no cross-reaction with CR2. The primary function of CD35 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This antibody labels follicular dendritic cells and follicular dendritic cell sarcoma. ELISA: Order BSA-free format for coating.
Presentation
1X PBS
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CR1 / CD35
Validation
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ CR1 / CD35 Antibody (clone E11, Azide-free, BSA-free) for IHC, IF/Immunofluorescence, Flow LS-B16937 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Species:
Human, Baboon, Monkey, Cynomolgus
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
Date Requested: 4/27/2024
