Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
Products
Antibodies
ELISA and Assay Kits
Research Areas
Infectious Disease
Resources
Purchasing
Reference Material
Contact Us
Locations
Orders Processing,
Shipping & Receiving,
Warehouse
2 Shaker Rd Suites
B001/B101
Shirley, MA 01464
Production Lab
Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036
Telephone Numbers
Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565
Contact Us
Additional Contact Details
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
| Catalog Number | Size | Price |
|---|---|---|
| LS-B7301-50 | 50 µg (1 mg/ml) | $440 |
PathPlus™ Monoclonal Mouse anti‑Human CR1 / CD35 Antibody (clone E11, IHC, WB) LS‑B7301
PathPlus™ Monoclonal Mouse anti‑Human CR1 / CD35 Antibody (clone E11, IHC, WB) LS‑B7301
Note: This antibody replaces LS-C11652, LS-C45217, LS-C11651
Antibody:
CR1 / CD35 Mouse anti-Human Monoclonal (E11) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CR1 / CD35 Mouse anti-Human Monoclonal (E11) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Specifications
Description
CD35 (also known as complement receptor type 1, CR1 or complement C3b/C4b receptor) is a membrane-bound protein member of the regulators of complement activation family. It mediates the immune adherence phenomenon; cellular binding to particles/immune complexes with activated complement. It also mediates inhibitory signals in B lymphocytes. CD35 is expressed at the cell surface in erythrocytes, dendritic cells, leukocytes, and podocytes. Polymorphisms and mutations of the CD35 gene have been reported in systemic lupus erythematosus, mesangiocapillary glomerulonephritis, gallbladder carcinoma, and sarcoidosis. Staining is used to identify dendritic cells and follicular dendritic cell tumors.
References: Immunol Res. 1987;6(3):192-209. PMID: 2960763; Proc R Soc Med. 1956;49(1):55-58. PMID: 13289834; J Immunol. 2002;168(6):2782-2788. PMID: 11884446; Mol Immunol. 2009;46(14)2767-2773. doi: 10.1016/j.molimm.2009.05.181; Hum Genet. 2005;118(2):225-234. doi: 10.1007/s00439-005-0044-6; Nephron. 1986;43(4):307. doi: 10.1159/000183861; Am J Surg Pathol. 1996;20(8):944-955. PMID: 8712294.
Target
Human CR1 / CD35
Synonyms
CR1 | C3b/C4b receptor | C3-binding protein | CD35 | C3BR | Knops blood group antigen | C4BR | CD35 antigen | Complement receptor type 1 | KN
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
E11
Purification
Protein A purified
Modifications
Unmodified.
Also available Preservative Free.
Immunogen
Human acute monocytic leukaemia cells.
Specificity
Recognizes CD35, a single chain cell surface glycoprotein which exists in four allotypic forms (A, B, C, D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement. Clone E11 has been reported to be suitable for use in Western Blot.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- IHC - Frozen (1 - 10 µg/ml)
- Western blot
- Flow Cytometry (1:50 - 1:200)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CR1 / CD35
Validation
Anti-CD35 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-CD35 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
PathPlus™ CR1 / CD35 Antibody (clone E11) for IHC, WB/Western, Flow LS-B7301 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC - Paraffin, Immunofluorescence, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Species:
Human, Baboon, Monkey, Cynomolgus
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Date Requested: 4/24/2024
