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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
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Catalog Number Size Price
LS-J1018-1 1 ea $268 
Product - Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).
Product - Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, Vector® SG substrate (blue/gray).
Product - Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin Labeling Kit and detected with VECTASTAIN® Elite® ABC Reagent and Vector® DAB substrate.
Product - Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).
Product - Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, Vector® SG substrate (blue/gray).
Product - Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin Labeling Kit and detected with VECTASTAIN® Elite® ABC Reagent and Vector® DAB substrate.
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VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Standard) - LS-J1018

VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Standard) - LS-J1018


Description:
This high-sensitivity Elite® ABC kit is for the detection of biotinylated antibodies and consists of Elite® Avidin DH and biotinylated Peroxidase. Approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit.
Price
Catalog Number
$268 
LS-J1018-1
1 ea

Available for shipment within the USA only
Toll Free North America
866-819-4732

Overview

Description:
This high-sensitivity Elite® ABC kit is for the detection of biotinylated antibodies and consists of Elite® Avidin DH and biotinylated Peroxidase. Approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit.

Specifications

Features: - Peroxidase enzyme detection system - Elite® Avidin/biotin complex (ABC) technology - High sensitivity/low background - Approximately 5 times more sensitive - Used as a second label in multiple antigen labeling applications - CE Mark - No antibody included - Concentrate Kit Contents: - 2 ml Reagent A (Elite® Avidin DH) plus 2 ml Reagent B (Biotinylated HRP) Applications: - Immunohistochemistry - Immunocytochemistry - In situ hybridization - Blotting Applications - Elispot - ELISA Details: The VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Standard) is part of the modular VECTASTAIN® ABC system (see the VECTASTAIN® ABC System document) , which can be used to detect any molecule that is biotinylated. Advanced avidin/biotin technology: The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target. The Elite® ABC series is approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background. This Standard kit consists of the Elite® HRP Enzyme Conjugate reagents listed in the following standard Immunohistochemistry protocol only, and is intended to be paired with biotinylated secondary antibodies, such as LS-D1 (goat anti-rabbit IgG), LS-D2 (horse anti-mouse IgG), or LS-D3 (horse anti-goat IgG). Alternatively, this kit is available pre-packaged with various combinations of species specific blocking serum and biotinylated secondary antibody (see VECTASTAIN® Elite® ABC-HRP Staining Kits LS-J1019 thought LS-J1025). Also available in Ready-To-Use (R.T.U.) formats: Prediluted, stabilized working solutions of Elite® ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN® Elite® ABC Staining Kit reagents (LS-J1026, LS-J1027, and LS-J1028). Immunohistochemistry protocol: Fixation – Histology - Antigen Retrieval – Endogenous Block – Serum Block -- Primary Antibody - Secondary Antibody – Enzyme Conjugate – Substrate - Counter-stain – Mounting


Restrictions
For research use only
Available for shipment within the USA only


LSBio is a proud distributor of Vector Laboratories products

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Images

Immunohistochemistry

Product - Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).
Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).

Immunohistochemistry

Product - Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, Vector® SG substrate (blue/gray).
Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, Vector® SG substrate (blue/gray).

Immunohistochemistry

Product - Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin Labeling Kit and detected with VECTASTAIN® Elite® ABC Reagent and Vector® DAB substrate.
Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin Labeling Kit and detected with VECTASTAIN® Elite® ABC Reagent and Vector® DAB substrate.

Immunohistochemistry

Product - Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).
Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).

Immunohistochemistry

Product - Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, Vector® SG substrate (blue/gray).
Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, Vector® SG substrate (blue/gray).

Immunohistochemistry

Product - Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin Labeling Kit and detected with VECTASTAIN® Elite® ABC Reagent and Vector® DAB substrate.
Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin Labeling Kit and detected with VECTASTAIN® Elite® ABC Reagent and Vector® DAB substrate.

Immunohistochemistry

Product - Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).
Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).

Immunohistochemistry

Product - Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, Vector® SG substrate (blue/gray).
Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, Vector® SG substrate (blue/gray).

Immunohistochemistry

Product - Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin Labeling Kit and detected with VECTASTAIN® Elite® ABC Reagent and Vector® DAB substrate.
Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin Labeling Kit and detected with VECTASTAIN® Elite® ABC Reagent and Vector® DAB substrate.

Immunohistochemistry

Product - Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).
Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).

Immunohistochemistry

Product - Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, Vector® SG substrate (blue/gray).
Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, Vector® SG substrate (blue/gray).

Immunohistochemistry

Product - Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin Labeling Kit and detected with VECTASTAIN® Elite® ABC Reagent and Vector® DAB substrate.
Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin Labeling Kit and detected with VECTASTAIN® Elite® ABC Reagent and Vector® DAB substrate.