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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Rat IgG) - LS-J1022

Available for shipment within the USA only
Catalog
Size
Price
LS-J1022-1
1 ea
$445

Available for shipment within the USA only

Product Description

This high-sensitivity Elite® ABC kit is for the detection of rat IgG primary antibodies and is sufficient for staining approximately 500-1000 sections. Contents include rabbit blocking serum, a biotinylated rabbit anti-rat IgG secondary antibody, Avidin DH, and biotinylated Peroxidase. Approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit.

Specifications

Details
Features: - Rat IgG primary antibody detection - Peroxidase enzyme detection system - Advanced Elite® Avidin/Biotin technology - Highest sensitivity, low background - Approximately 5 times more sensitive - Format: Concentrate - Used as a second label in multiple antigen labeling applications - Sufficient for staining approximately 500-1000 sections Kit Contents: - 3 ml Normal Rabbit Serum - 1 ml Biotinylated Rabbit Anti-Rat IgG - 2 ml Reagent A (Avidin DH) plus 2 ml Reagent B (Biotinylated Peroxidase) Applications: - Immunohistochemistry - Immunocytochemistry - In situ hybridization - Blotting Applications - Elispot - ELISA Details: The VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Rat IgG) is part of the modular VECTASTAIN® ABC system (see the VECTASTAIN® ABC System document) , which can be used to detect any molecule that is biotinylated. Advanced avidin/biotin technology: The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target. The Elite® ABC series is approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background. This kit consists of the Blocking Serum, a species specific Biotinylated Secondary Antibody, and the Elite® HRP Enzyme Conjugate reagents listed in the following standard Immunohistochemistry protocol. Alternatively, this kit is also available without the Blocking Serum or species-specific Biotinylated Secondary Antibody (LS-J1018). Immunohistochemistry protocol: Fixation – Histology - Antigen Retrieval – Endogenous Block – Serum Block -- Primary Antibody - Secondary Antibody – Enzyme Conjugate – Substrate - Counter-stain – Mounting
Restrictions
For research use only. Available for shipment within the USA only.

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Requested From: United States
Date Requested: 3/19/2019
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