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VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal), R.T.U. (Ready-to-Use) - LS-J1027
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This high-sensitivity Elite® ABC kit is for the detection of mouse and rabbit IgG primary antibodies and is sufficient for staining approximately 250-500 sections. Contents include Ready-to-Use rabbit blocking serum, biotinylated horse anti-mouse, and rabbit IgG secondary antibody, Avidin DH, and biotinylated Peroxidase. Approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit.
Specifications
Details
Features:
- Ready-to-Use
- Mouse and Rabbit IgG primary antibody detection
- Peroxidase enzyme detection system
- Advanced Elite® Avidin/Biotin technology
- Highest sensitivity, low background
- Approximately 5 times more sensitive
- Used as a second label in multiple antigen labeling applications
- Sufficient for staining approximately 250-500 sections
Kit Contents:
- 50 ml Normal Horse Serum
- 50 ml Biotinylated Horse Anti-Mouse and Rabbit IgG
- 50 ml Stabilized Elite® ABC Reagent (Elite® Avidin DH and Biotinylated Peroxidase)
Applications:
- Immunohistochemistry
- Immunocytochemistry
- In situ hybridization
- Blotting Applications
- Elispot
- ELISA
Details:
The VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal IgG) is part of the modular VECTASTAIN® ABC system (see the VECTASTAIN® ABC System document) , which can be used to detect any molecule that is biotinylated.
Advanced avidin/biotin technology: The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target. The Elite® ABC series is approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background.
This kit consists of the Blocking Serum, a species specific Biotinylated Secondary Antibody, and the Elite® HRP Enzyme Conjugate reagents listed in the following standard Immunohistochemistry protocol. Alternatively, this kit is also available without the Blocking Serum or species-specific Biotinylated Secondary Antibody (LS-J1018).
Immunohistochemistry protocol:
Fixation – Histology - Antigen Retrieval – Endogenous Block – Serum Block -- Primary Antibody - Secondary Antibody – Enzyme Conjugate – Substrate - Counter-stain – Mounting
Documents
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Restrictions
For research use only.
Available for shipment within the USA only.
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