- Peroxidase enzyme detection system
- Elite® Avidin/biotin complex (ABC) technology
- No antibody included
- High sensitivity/low background
- Approximately 5 times more sensitive
- Contains sufficient reagent to stain approximately 250-500 sections
- Used as a second label in multiple antigen labeling applications
- 50 ml ready-to-use stabilized Elite® ABC reagent
- In situ hybridization
- Blotting Applications
The VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Standard) is part of the modular VECTASTAIN® ABC system (see the VECTASTAIN® ABC System document) , which can be used to detect any molecule that is biotinylated.
Advanced avidin/biotin technology: The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target. The Elite® ABC series is approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background.
This Standard kit consists of the Ready-to-Use formulation of the Elite® HRP Enzyme Conjugate reagents listed in the following standard Immunohistochemistry protocol only, and is intended to be paired with biotinylated secondary antibodies, such as LS-D1 (goat anti-rabbit IgG), LS-D2 (horse anti-mouse IgG), or LS-D3 (horse anti-goat IgG). Alternatively, this kit is available pre-packaged with various combinations of species specific blocking serum and biotinylated secondary antibody (see VECTASTAIN® Elite® ABC-HRP Staining Kits LS-J1019 thought LS-J1025).
Fixation – Histology - Antigen Retrieval – Endogenous Block – Serum Block -- Primary Antibody - Secondary Antibody – Enzyme Conjugate – Substrate - Counter-stain – Mounting
For research use only.
Available for shipment within the USA only.