Features: - Peroxidase enzyme detection system - Elite® Avidin/biotin complex (ABC) technology - Ready-to-Use - No antibody included - High sensitivity/low background - Approximately 5 times more sensitive - Contains sufficient reagent to stain approximately 250-500 sections - Used as a second label in multiple antigen labeling applications Kit Contents: - 50 ml ready-to-use stabilized Elite® ABC reagent Applications: - Immunohistochemistry - Immunocytochemistry - In situ hybridization - Blotting Applications - Elispot - ELISA Details: The VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Standard) is part of the modular VECTASTAIN® ABC system (see the VECTASTAIN® ABC System document) , which can be used to detect any molecule that is biotinylated. Advanced avidin/biotin technology: The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target. The Elite® ABC series is approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background. This Standard kit consists of the Ready-to-Use formulation of the Elite® HRP Enzyme Conjugate reagents listed in the following standard Immunohistochemistry protocol only, and is intended to be paired with biotinylated secondary antibodies, such as LS-D1 (goat anti-rabbit IgG), LS-D2 (horse anti-mouse IgG), or LS-D3 (horse anti-goat IgG). Alternatively, this kit is available pre-packaged with various combinations of species specific blocking serum and biotinylated secondary antibody (see VECTASTAIN® Elite® ABC-HRP Staining Kits LS-J1019 thought LS-J1025). Immunohistochemistry protocol: Fixation – Histology - Antigen Retrieval – Endogenous Block – Serum Block -- Primary Antibody - Secondary Antibody – Enzyme Conjugate – Substrate - Counter-stain – Mounting