Osteopontin antibody LS-B425 is an unconjugated rabbit polyclonal antibody to Osteopontin (SPP1) from human, mouse, rat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Human SPP1 / Osteopontin
Human, Mouse, Rat, Dog, Pig
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:500)
- Western blot (1:500 - 1:2000)
- ELISA (1:20000 - 1:100000)
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Synthetic peptide from human SPP1 / Osteopontin.
Synthetic peptide C-K-S-K-K-F-R-R-P-D-I-Q-Y-P-D corresponding to human osteopontin proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Immunohistochemistry: LS-B425 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B425 was determined to be 1:500.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.