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SPP1 / Osteopontin Antibody (N-Terminus) IHC-plus™ LS-B425

Note: This antibody replaces LS-C19062
Anti-Osteopontin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Rabbit anti-Osteopontin was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.
Anti-Osteopontin Antibody - Western Blot. Rabbit anti-Osteopontin was used at a 1:1000 dilution to detect Osteopontin by western blot. In lane 2, reactivity is shown against 250 ng of human osteopontin. Lane 3 shows reactivity of MMP-cleaved osteopontin. Lane 1 shows the position of molecular weight markers. Use a 1:10000 dilution of HRP conjugated Gt-a-Rabbit IgG (LS-C60865) for detection.
Anti-Osteopontin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Rabbit anti-Osteopontin was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.
Anti-Osteopontin Antibody - Western Blot. Rabbit anti-Osteopontin was used at a 1:1000 dilution to detect Osteopontin by western blot. In lane 2, reactivity is shown against 250 ng of human osteopontin. Lane 3 shows reactivity of MMP-cleaved osteopontin. Lane 1 shows the position of molecular weight markers. Use a 1:10000 dilution of HRP conjugated Gt-a-Rabbit IgG (LS-C60865) for detection.
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SPP1 / Osteopontin Antibody (N-Terminus) IHC-plus™ LS-B425

Note: This antibody replaces LS-C19062
Antibody:
Rabbit Polyclonal to Human SPP1 / Osteopontin
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat, Dog, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B425-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human SPP1 / Osteopontin
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat, Dog, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
Osteopontin antibody LS-B425 is an unconjugated rabbit polyclonal antibody to Osteopontin (SPP1) from human, mouse, rat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.

Target
Human SPP1 / Osteopontin
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Sterile filtered
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:500)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:20000 - 1:100000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human SPP1 / Osteopontin.
Epitope
N-Terminus
Specificity
Synthetic peptide C-K-S-K-K-F-R-R-P-D-I-Q-Y-P-D corresponding to human osteopontin proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Usage
Immunohistochemistry: LS-B425 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B425 was determined to be 1:500.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPP1 / Osteopontin
P10451 NM_000582 NP_000573.1

Validation

Anti-Osteopontin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
IHC Validated
Anti-Osteopontin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-Osteopontin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

Publications (2)

Osteopontin induces beta-catenin signaling through activation of Akt in prostate cancer cells. Robertson BW, Chellaiah MA. Experimental cell research. 2010 316:11-Jan.

Effects of Cryopreservation on Canine Multipotent Stromal Cells from Subcutaneous and Infrapatellar Adipose Tissue. Duan W, Lopez MJ. Stem cell reviews. 2016 12:257-68. (ICC, Flo; Canine)

Reviews (0)

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Requested From: United States
Date Requested: 6/27/2019
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