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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPP1 / Osteopontin

secreted phosphoprotein 1

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

Gene Name: secreted phosphoprotein 1
Synonyms: SPP1, BSPI, BNSP, Bone sialoprotein 1, ETA-1, Nephropontin, OPN, Secreted phosphoprotein 1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin, Osteopontin, Bone sialoprotein I
Target Sequences: NM_000582 NP_000573.1 P10451

Publications (5)

1
Mapping of functional epitopes of osteopontin by monoclonal antibodies raised against defined internal sequences. Kon S, Yokosaki Y, Maeda M, Segawa T, Horikoshi Y, Tsukagoshi H, Rashid MM, Morimoto J, Inobe M, Shijubo N, Chambers AF, Uede T. Journal of cellular biochemistry. 2002 84:420-32. [PubMed:11787071]
2
Osteopontin induces beta-catenin signaling through activation of Akt in prostate cancer cells. Robertson BW, Chellaiah MA. Experimental cell research. 2010 316:11-Jan. [PubMed:19850036] [PMC:PMC2787770] Related Antibodies: LS-B425.
3
Expression of chondro-osteogenic BMPs in clinical samples of patellar tendinopathy. Rui YF, Lui PP, Rolf CG, Wong YM, Lee YW, Chan KM. Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA. 2012 20:1409-17. (IHC; Human) [PubMed:21946950]
4
Cellular retinol-binding protein 1 (CRBP-1) regulates osteogenenesis and adipogenesis of mesenchymal stem cells through inhibiting RXR-induced -catenin degradation. Xu L, Song C, Ni M, Meng F, Xie H, Li G. The international journal of biochemistry & cell biology. 2012 44:612-9. [PubMed:22230368]
5
Effects of Cryopreservation on Canine Multipotent Stromal Cells from Subcutaneous and Infrapatellar Adipose Tissue. Duan W, Lopez MJ. Stem cell reviews. 2016 12:257-68. (ICC, Flo; Canine) [Full Text Article] [PubMed:26537238] [PMC:PMC4841859] Related Antibodies: LS-B425.
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Filtered by: None
Target
SPP1 / Osteopontin (172)
Reactivity
Human (152)
Mouse (64)
Rat (33)
Bovine (4)
Dog (15)
Gibbon (3)
Guinea pig (8)
Horse (4)
Monkey (9)
Orangutan (6)
Pig (15)
Rabbit (10)
Application
IHC (57)
IHC-Fr (1)
IHC-P (32)
WB (156)
Flo (7)
ELISA (86)
ICC (12)
IF (12)
IP (7)
Peptide-ELISA (2)
Host
rabbit (101)
mouse (64)
goat (2)
chicken (5)
Isotype
IgG (44)
IgG1 (48)
IgG1,k (6)
IgG2a (5)
IgG2a,k (2)
IgG2b (1)
IgG2b,k (1)
Clonality
monoclonal mc (64)
polyclonal pc (108)
Clone
10A16 (1)
11n145 (2)
12K149 (1)
14C08 (1)
1B20 (5)
1F11 (2)
2C5 (2)
2E11 (2)
2F10 (2)
2F2 (1)
2H9 (2)
34E3 (1)
3C4 (1)
53 (1)
5E4 (1)
6A12 (1)
6C12 (1)
7C5H12 (2)
87-B (1)
X1 (1)
Format
AP Conjugated (6)
APC (4)
APC Conjugated (11)
Azide-free (1)
Biotin Conjugated (18)
CF Conjugated (1)
Cy3 Conjugated (4)
Cy7 Conjugated (4)
DY488 Conjugated (1)
DY550 Conjugated (1)
DY650 Conjugated (1)
FITC Conjugated (16)
HRP Conjugated (15)
PE Conjugated (12)
Unconjugated (82)
Epitope
C-Terminus (8)
N-Terminus (8)
aa14-40 (8)
aa17-287 (8)
aa18-314 (8)
aa7-232 (8)
aa17-314 (7)
aa17-300 (3)
230-310 aa, C-terminal (1)
Amino Acid: 167-314 (1)
Internal (1)
Leu285 (1)
aa164-179 (1)
aa261-310 (1)
aa273-301 (1)
aa28-47 (1)
aa281-314 (1)
aa288-299 (1)
aa59-74 (1)
aa75-90 (1)
Publications
No (171)
Yes (1)
Showing 1-25 of 172 results

SPP1 / Osteopontin Antibody (N-Terminus) IHC-plus™ LS-B425
Publications (2)
Publications (2)
Rabbit Polyclonal to Human SPP1 / Osteopontin
Mouse, Dog, Rat, Pig, Human
ELISA IHC IHC-P WB
Unconjugated
50µl 82mg/ml  $425.00
Anti-Osteopontin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Rabbit anti-Osteopontin was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.

SPP1 / Osteopontin Antibody (C-Terminus, clone 1B20) IHC-plus™ LS-B8326
Mouse Monoclonal [clone 1B20] (IgG1) to Human SPP1 / Osteopontin
Rabbit, Rat, Human
ICC IHC IHC-P IP WB
Unconjugated
100µl 1mg/ml  $425.00
Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Osteopontin Antibody (1B20) - IHC of Osteopontin on human lung adenocarcinoma.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

SPP1 / Osteopontin Antibody (clone 1B20, Biotin) LS-C180345
Mouse Monoclonal [clone 1B20] (IgG1) to Human SPP1 / Osteopontin
Rabbit, Rat, Human
ICC IHC IHC-P IP WB
Biotin Conjugated
100µl 0.8mg/ml  $365.00
Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Osteopontin Antibody (1B20) - IHC of Osteopontin on human lung adenocarcinoma.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

SPP1 / Osteopontin Antibody IHC-plus™ LS-B10122
Rabbit Polyclonal (IgG) to Human SPP1 / Osteopontin
Mouse, Rat, Human
ELISA IF IHC IHC-P WB
Unconjugated
0.05mg 1mg/ml  $425.00
Anti-SPP1 / Osteopontin antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Immunofluorescence of SPP1 in human bladder tissue with SPP1 antibody at 20 ug/ml.

SPP1 / Osteopontin Antibody (aa288-299) IHC-plus™ LS-B2411
Goat Polyclonal to Human SPP1 / Osteopontin
Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µg 0.5mg/ml  $425.00
Anti-Osteopontin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Staining (0.3 ug/ml) of Human Thymus lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by using chemiluminescence.

SPP1 / Osteopontin Antibody (C-Terminus) LS-C31763
Rabbit Polyclonal (IgG) to Human SPP1 / Osteopontin
Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Human
IHC IHC-P WB
Unconjugated
100µl 0.5mg/ml  $335.00
SPP1 / Osteopontin antibody ARP36677_T100-NP_000573-SPP1 Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

SPP1 / Osteopontin Antibody (clone 2C5) LS-C305906
Mouse Monoclonal [clone 2C5] (IgG1,k) to Human SPP1 / Osteopontin
Human
ELISA WB
Unconjugated
100µg  $425.00
Western blot of SPP1 / Osteopontin antibody LS-X223133.

SPP1 / Osteopontin Antibody (clone 2F10) LS-C305907
Mouse Monoclonal [clone 2F10] (IgG1,k) to Human SPP1 / Osteopontin
Human
ELISA WB
Unconjugated
100µg 1mg/ml  $445.00
Western blot of SPP1 / Osteopontin antibody LS-X223134.

SPP1 / Osteopontin Antibody (aa14-40) LS-C323539
Rabbit Polyclonal (IgG) to Human SPP1 / Osteopontin
Human
ELISA IF IHC WB
Unconjugated
200µl  $590.00

SPP1 / Osteopontin Antibody (clone 1F11) LS-C305911
Mouse Monoclonal [clone 1F11] (IgG1,k) to Human SPP1 / Osteopontin
Human
ELISA WB
Unconjugated
100µg  $425.00
Western blot of SPP1 / Osteopontin antibody LS-X223137.

SPP1 / Osteopontin Antibody (clone X1) IHC-plus™ LS-B5940
Mouse Monoclonal [clone X1] (IgG2b,k) to Human SPP1 / Osteopontin
Human
ELISA IHC IHC-P WB
Unconjugated
50µg  $495.00
Anti-Osteopontin antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Detection limit for recombinant GST tagged SPP1 is approximately 0.3 ng/ml as a capture antibody.

SPP1 / Osteopontin Antibody (aa14-40) IHC-plus™ LS-B9287
Rabbit Polyclonal to Human SPP1 / Osteopontin
Human
IF IHC IHC-P WB
Unconjugated
200µl  $425.00
Anti-SPP1 / Osteopontin antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Confocal immunofluorescence of OPN-a/b Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

SPP1 / Osteopontin Antibody (aa273-301) LS-C99283
Rabbit Polyclonal to Human SPP1 / Osteopontin
Human
Flo IHC IHC-P WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human lung carcinoma with SPP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of SPP1 Antibody in NCI-H460 cell line lysates (35 ug/lane). SPP1 (arrow) was detected using the purified antibody.

SPP1 / Osteopontin Antibody (PE) LS-C131160
Goat Polyclonal (IgG) to Mouse SPP1 / Osteopontin
Mouse
Flo
PE Conjugated
100tst  $650.00

SPP1 / Osteopontin Antibody LS-C96393
Rabbit Polyclonal to Mouse SPP1 / Osteopontin
Mouse
IHC IHC-Fr IHC-P
Unconjugated
0.5ml  $465.00

SPP1 / Osteopontin Antibody (clone 2E11) LS-C189637
Mouse Monoclonal [clone 2E11] (IgG1) to Human SPP1 / Osteopontin
Mouse, Human
ELISA WB
Unconjugated
1mg  $710.00

SPP1 / Osteopontin Antibody (aa17-314) LS-C137501
Mouse Monoclonal (IgG1) to Human SPP1 / Osteopontin
Human
ELISA WB
Unconjugated
100µl 1mg/ml  $385.00
Western blot Image

SPP1 / Osteopontin Antibody (clone 7C5H12) LS-C169155
Mouse Monoclonal [clone 7C5H12] (IgG1) to Human SPP1 / Osteopontin
Human
ELISA IF IHC WB
Unconjugated
100µg 1mg/ml  $365.00
IHC of paraffin-embedded prostate cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded endometrial cancer tissues using SPP1 mouse monoclonal antibody with DAB staining.

SPP1 / Osteopontin Antibody (aa261-310) IHC-plus™ LS-B9080
Rabbit Polyclonal (IgG) to Human SPP1 / Osteopontin
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
100µl 1mg/ml  $505.00
Anti-SPP1 / Osteopontin antibody IHC staining of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Western blot analysis of lysates from LOVO cells, using Osteopontin Antibody. The lane on the right is blocked with the synthesized peptide.

SPP1 / Osteopontin Antibody (Internal) IHC-plus™ LS-B5272
Rabbit Polyclonal (IgG) to Human SPP1 / Osteopontin
Human
IHC IHC-P WB
Unconjugated
250µl  $425.00
Anti-Osteopontin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:25.

SPP1 / Osteopontin Antibody LS-C9413
Rabbit Polyclonal to Human SPP1 / Osteopontin
Human
ELISA IHC IHC-P WB
Unconjugated
100µl  $485.00

SPP1 / Osteopontin Antibody (aa59-74) LS-C122259
Rabbit Polyclonal to Human SPP1 / Osteopontin
Human
ELISA IHC IHC-P WB
Unconjugated
20µl  $425.00; 100µl  $890.00

SPP1 / Osteopontin Antibody (N-Terminus) IHC-plus™ LS-B5626
Rabbit Polyclonal to Human SPP1 / Osteopontin
Rabbit, Guinea pig, Horse, Human
IHC IHC-P WB
Unconjugated
100µl  $485.00
Anti-Osteopontin antibody IHC of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPP1 / Osteopontin antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

SPP1 / Osteopontin Antibody (aa75-90) LS-C9410
Rabbit Polyclonal (IgG) to Human SPP1 / Osteopontin
Human
ELISA IHC IHC-P
Unconjugated
100µl  $785.00

SPP1 / Osteopontin Antibody LS-C136850
Rabbit Polyclonal (IgG) to Human SPP1 / Osteopontin
Human
ELISA WB
Unconjugated
100µl  $275.00
Western blot Image
Showing 1-25 of 172 results

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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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