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Catalog Number Size Price
LS-B2411-50 50 µg (0.5 mg/ml) $485 
SPP1 / Osteopontin Antibody - Anti-Osteopontin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
SPP1 / Osteopontin Antibody - Osteopontin / SPP1 Antibody (0.1µg/ml) staining of lysates of cell line MOLT4 (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
SPP1 / Osteopontin Antibody - SPP1 / Osteopontin antibody (0.5µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
SPP1 / Osteopontin Antibody - Staining (0.3 ug/ml) of Human Thymus lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by using chemiluminescence.
SPP1 / Osteopontin Antibody - Anti-Osteopontin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
SPP1 / Osteopontin Antibody - Osteopontin / SPP1 Antibody (0.1µg/ml) staining of lysates of cell line MOLT4 (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
SPP1 / Osteopontin Antibody - SPP1 / Osteopontin antibody (0.5µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
SPP1 / Osteopontin Antibody - Staining (0.3 ug/ml) of Human Thymus lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by using chemiluminescence.
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IHC‑plus™ Polyclonal Goat anti‑Human SPP1 / Osteopontin Antibody (aa288‑299, IHC, WB) LS‑B2411

IHC‑plus™ Polyclonal Goat anti‑Human SPP1 / Osteopontin Antibody (aa288‑299, IHC, WB) LS‑B2411

Note: This antibody replaces LS-C20186, LS-C73948
Antibody:
SPP1 / Osteopontin Goat anti-Human Polyclonal (aa288-299) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B2411-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SPP1 / Osteopontin Goat anti-Human Polyclonal (aa288-299) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Osteopontin antibody LS-B2411 is an unconjugated goat polyclonal antibody to human Osteopontin (SPP1) (aa288-299). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SPP1 / Osteopontin
Synonyms
SPP1 | BSPI | BNSP | Bone sialoprotein 1 | ETA-1 | Nephropontin | OPN | Secreted phosphoprotein 1 | SPP-1 | SPP1/CALPHA1 fusion | Urinary stone protein | Uropontin | Osteopontin | Bone sialoprotein I
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-DPKSKEEDKHLK, from the C-Terminus of protein sequence according to NP_001035147.1NP_000573.1NP_001035149.1.
Epitope
aa288-299
Specificity
Human SPP1 / Osteopontin.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2411 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2411 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPP1 / Osteopontin
P10451 NM_000582 NP_000573.1

Validation

SPP1 / Osteopontin Antibody - Anti-Osteopontin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Osteopontin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Osteopontin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ SPP1 / Osteopontin Antibody (aa288-299) for IHC, WB/Western LS-B2411 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/21/2024