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| Catalog Number | Size | Price |
|---|---|---|
| LS-C744596-25 | 25 µl (75 mg/ml) | $304 |
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Polyclonal Rabbit anti‑Human SPP1 / Osteopontin Antibody (IHC, WB) LS‑C744596
Polyclonal Rabbit anti‑Human SPP1 / Osteopontin Antibody (IHC, WB) LS‑C744596
Antibody:
SPP1 / Osteopontin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat, Dog, Pig
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
SPP1 / Osteopontin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat, Dog, Pig
Format:
Unconjugated, Unmodified
Specifications
Description
Osteopontin antibody LS-C744596 is an unconjugated rabbit polyclonal antibody to Osteopontin (SPP1) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB.
Target
Human SPP1 / Osteopontin
Synonyms
SPP1 | BSPI | BNSP | Bone sialoprotein 1 | ETA-1 | Nephropontin | OPN | Secreted phosphoprotein 1 | SPP-1 | SPP1/CALPHA1 fusion | Urinary stone protein | Uropontin | Osteopontin | Bone sialoprotein I
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Pig
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, from the human osteopontin protein, conjugated to KLH using maleimide.
Specificity
The antibody recognizes the full-length osteopontin protein (which runs at 66 kDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 kDa MMP-cleaved C-fragment is recognized, but not the 40 kDa N-terminal fragment. Reactivity is reported to occur with osteopontin from swine, dog, mouse and rat.
Applications
- IHC (1:100 - 1:300)
- Western blot (1:500 - 1:2000)
- Immunoprecipitation (1:100)
- ELISA (1:5000 - 1:20000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SPP1 / Osteopontin
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat, Dog, Pig
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Species:
Human, Rat, Rabbit
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/23/2024
Date Requested: 5/23/2024
