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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPHK / SPHK1 Antibody (N‑Terminus) IHC‑plus™ LS‑A3102
SPHK1 antibody LS-A3102 is an unconjugated rabbit polyclonal antibody to human SPHK1 (SPHK). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A3102-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment

Popular SPHK / SPHK1 Products

Anti-SPHK1 antibody LS-A3106 IHC of human adrenal, pheochromocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin
Western blot of anti-mouse SphK1 (pS225) (LS-C26925) on mouse B-cell lysate. Antibody used at 1 ug/ml with phosphorylated blocking peptideNo. X1877B) (lane A) and without (lane B).
Species: Mouse
Applications: Western blot
Antibody (2 ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Species: Human, Monkey, Mouse, Hamster
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human, Mouse
Applications: Western blot, Flow Cytometry

Product Description

SPHK1 antibody LS-A3102 is an unconjugated rabbit polyclonal antibody to human SPHK1 (SPHK). Validated for IHC. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human SPHK / SPHK1
Synonyms
SPHK1, SK1, Sphingosine kinase 1, SPHK, SK 1, SPK 1, Sphingosine kinase type 1, SPK
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SPHK / SPHK1 antibody was raised against synthetic 15 amino acid peptide from N-terminus of human SPHK1. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (93%); Bat, Pig (87%); Mouse, Rat, Hamster (80%).
Blocking Peptide
LS-E28686 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A3102. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human SPHK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A3102 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A3102 was determined to be 20 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SPHK / SPHK1
SPHK / SPHK1 catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Q9NYA1 NM_021972 NP_068807.2


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Images

Immunohistochemistry

Anti-SPHK1 antibody LS-A3102 IHC of human adrenal, zona glomerulosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SPHK1 antibody LS-A3102 IHC of human adrenal, zona glomerulosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-SPHK1 antibody LS-A3102 IHC of human adrenal, zona glomerulosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SPHK1 antibody LS-A3102 IHC of human adrenal, zona glomerulosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-SPHK1 antibody LS-A3102 IHC of human adrenal, zona glomerulosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SPHK1 antibody LS-A3102 IHC of human adrenal, zona glomerulosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-SPHK1 antibody LS-A3102 IHC of human adrenal, zona glomerulosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SPHK1 antibody LS-A3102 IHC of human adrenal, zona glomerulosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 10/15/2018

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