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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPHK / SPHK1

sphingosine kinase 1

SPHK / SPHK1 catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Name: sphingosine kinase 1
Family/Subfamily: Non-protein Kinase , Sphingosine
Synonyms: SPHK1, SK1, Sphingosine kinase 1, SPHK, SK 1, SPK 1, Sphingosine kinase type 1, SPK
Target Sequences: NM_021972 NP_068807.2 Q9NYA1

Publications (1)

1
Sphingosine kinase 1 and sphingosine 1-phosphate receptor 3 are functionally upregulated on astrocytes under pro-inflammatory conditions. Fischer I, Alliod C, Martinier N, Newcombe J, Brana C, Pouly S. PloS one. 2011 6:e23905. [PubMed:21887342] [PMC:PMC3161076] Related Antibodies: LS-C100769.

Filtered by: None
Target
SPHK / SPHK1 (81)
Reactivity
Human (77)
Mouse (34)
Rat (16)
Bovine (1)
Chimpanzee (3)
Gibbon (3)
Guinea pig (4)
Hamster (2)
Horse (4)
Monkey (28)
Pig (1)
Primate (1)
Application
IHC (42)
IHC-P (27)
WB (55)
Flo (2)
ELISA (21)
ICC (2)
IF (6)
IP (4)
Peptide-ELISA (1)
Host
rabbit (78)
mouse (2)
goat (1)
Isotype
IgG (31)
IgG2a (2)
Clonality
monoclonal mc (2)
polyclonal pc (79)
Clone
OTI1A6 (2)
Format
AP Conjugated (5)
APC (1)
APC Conjugated (6)
Azide-free (3)
Biotin Conjugated (8)
Carrier-free (1)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (9)
HRP Conjugated (8)
PE Conjugated (6)
Unconjugated (37)
Epitope
N-Terminus (21)
aa1-30 (8)
aa148-398 (8)
aa286-315 (8)
aa176-225 (4)
aa334-384 (2)
C-Terminus (1)
aa1-50 (1)
aa117-133 (1)
aa176-189 (1)
aa313-343 (1)
aa59-89 (1)
pSer225 (1)
Publications
No (80)
Yes (1)
Showing 1-25 of 81 results

IHC-plus™ SPHK / SPHK1 Antibody (N-Terminus) LS-A3106
Rabbit Polyclonal to Human SPHK / SPHK1
Human, Monkey
IHC, IHC-P
Unconjugated
50µg 50µl 1mg/ml  $425.00
Anti-SPHK1 antibody IHC of human adrenal, pheochromocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ SPHK / SPHK1 Antibody LS-B6973
Rabbit Polyclonal (IgG) to Human SPHK / SPHK1
Mouse, Hamster, Human, Monkey
ICC, IHC, IHC-P, WB
Unconjugated
1ea  $425.00
Anti-SPHK1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western blot of SPHK / SPHK1 antibody.

SPHK / SPHK1 Antibody (phospho-Ser225) LS-C26925
Rabbit Polyclonal to Mouse SPHK / SPHK1
Mouse
WB
Unconjugated
100µg 0.5mg/ml  $485.00
Western blot of anti-mouse SphK1 (pS225) (phospho-Sphingosine Kinase 1,(pS225), Mouse reactive) on mouse B-cell lysate. Antibody used at 1 ug/ml with phosphorylated blocking peptideNo. X1877B) (lane A) and without (lane B).

SPHK / SPHK1 Antibody (aa286-315) LS-C100769
Publications (1)
Publications (1)
Rabbit Polyclonal to Human SPHK / SPHK1
Mouse, Rat, Primate, Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00; 200µl  $325.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of lysates from 293, HepG2, HUVEC, Raji, rat C6, mouse C2C12 cell line (from left to right), using SPHK1 Antibody (R301). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

SPHK / SPHK1 Antibody (aa176-189) LS-C87246
Goat Polyclonal to Human SPHK / SPHK1
Mouse, Hamster, Human, Monkey
IP, Peptide-ELISA, WB
Unconjugated
100µg 0.5mg/ml  $405.00
Antibody (2 ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

IHC-plus™ SPHK / SPHK1 Antibody (N-Terminus) LS-A3102
Rabbit Polyclonal to Human SPHK / SPHK1
Human
IHC, IHC-P
Unconjugated
50µg 50µl 1mg/ml  $425.00
Anti-SPHK1 antibody IHC of human adrenal, zona glomerulosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

SPHK / SPHK1 Antibody (FITC) LS-C146698
Rabbit Polyclonal (IgG) to Human SPHK / SPHK1
Mouse, Human
Flo, WB
FITC Conjugated
500µl  $365.00

SPHK / SPHK1 Antibody (aa313-343) LS-C161448
Rabbit Polyclonal to Human SPHK / SPHK1
Human
Flo, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
SPHK1 Antibody (C-term R414)IHC of formalin-fixed and paraffin-embedded skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of the SPHK1 Antibody (C-term R414) for immunohistochemistry.
Western blot of SPHK1 Antibody (C-term R414) in 293 cell line lysates (35 ug/lane). SPHK1 (arrow) was detected using the purified antibody.

SPHK / SPHK1 Antibody LS-C330808
Rabbit Polyclonal (IgG) to Human SPHK / SPHK1
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded human gastric cancer using SPHK1 Antibodyat dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using SPHK1 Antibodyat dilution of 1:100 (40x lens).

SPHK / SPHK1 Antibody (aa59-89) LS-C100770
Rabbit Polyclonal to Human SPHK / SPHK1
Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Immunohistochemical of paraffin-embedded H. kidney section using SPHK1 Antibody(N-term P74). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical of paraffin-embedded M. kidney section using SPHK1 Antibody(N-term P74). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

SPHK / SPHK1 Antibody (clone OTI1A6) LS-C338427
Mouse Monoclonal [clone OTI1A6] (IgG2a) to Human SPHK / SPHK1
Human
IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPHK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human endometrium tissue using anti-SPHK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

SPHK / SPHK1 Antibody (clone OTI1A6, Carrier-free) LS-C788509
Mouse Monoclonal [clone OTI1A6] (IgG2a) to Human SPHK / SPHK1
Human
IF, IHC, WB
Unconjugated, Carrier-free
100µg  $395.00

SPHK / SPHK1 Antibody LS-C152974
Rabbit Polyclonal to Human SPHK / SPHK1
Human
IHC
Unconjugated
50µg  $515.00

SPHK / SPHK1 Antibody LS-C499856
Rabbit Polyclonal (IgG) to Human SPHK / SPHK1
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human liver cancer tissue using SPHK1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using SPHK1 Antibody at dilution of 1:100

SPHK / SPHK1 Antibody (aa117-133) LS-C313130
Rabbit Polyclonal to Human SPHK / SPHK1
Pig, Human, Monkey
ICC, IHC, IHC-P, WB
Unconjugated
100µg 200µl 0.5mg/ml  $305.00
SPHK / SPHK1 antibody. IHC(P): Human Placenta Tissue.
SPHK / SPHK1 antibody. ICC: HELA Cell.

IHC-plus™ SPHK / SPHK1 Antibody (N-Terminus) LS-A3104
Rabbit Polyclonal to Human SPHK / SPHK1
Human, Monkey
IHC, IHC-P
Unconjugated
50µg 50µl 1mg/ml  $425.00
Anti-SPHK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SPHK1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ SPHK / SPHK1 Antibody (N-Terminus, Biotin) LS-A103615
Rabbit Polyclonal to Human SPHK / SPHK1
Human, Monkey
IHC, IHC-P
Biotin Conjugated
100µl  $650.00
Anti-SPHK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SPHK1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ SPHK / SPHK1 Antibody (N-Terminus, FITC) LS-A103616
Rabbit Polyclonal to Human SPHK / SPHK1
Human, Monkey
IHC, IHC-P
FITC Conjugated
100µl  $650.00
Anti-SPHK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SPHK1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ SPHK / SPHK1 Antibody (N-Terminus, PE) LS-A103618
Rabbit Polyclonal to Human SPHK / SPHK1
Human, Monkey
IHC, IHC-P
PE Conjugated
100µl  $650.00
Anti-SPHK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SPHK1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ SPHK / SPHK1 Antibody (N-Terminus, APC) LS-A103614
Rabbit Polyclonal to Human SPHK / SPHK1
Human, Monkey
IHC, IHC-P
APC Conjugated
100µl  $650.00
Anti-SPHK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SPHK1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ SPHK / SPHK1 Antibody (N-Terminus, Azide-free, HRP) LS-A103617
Rabbit Polyclonal to Human SPHK / SPHK1
Human, Monkey
IHC, IHC-P
HRP Conjugated, Azide-free
100µl  $650.00
Anti-SPHK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SPHK1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ SPHK / SPHK1 Antibody (N-Terminus, AP) LS-A103613
Rabbit Polyclonal to Human SPHK / SPHK1
Human, Monkey
IHC, IHC-P
AP Conjugated
100µl  $650.00
Anti-SPHK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SPHK1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

SPHK / SPHK1 Antibody LS-C805942
Rabbit Polyclonal (IgG) to Human SPHK / SPHK1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00

SPHK / SPHK1 Antibody (aa286-315) LS-C325332
Rabbit Polyclonal (IgG) to Human SPHK / SPHK1
Mouse, Rat, Human, Monkey
ELISA, IHC, WB
Unconjugated
200µl  $590.00

SPHK / SPHK1 Antibody LS-C751895
Rabbit Polyclonal (IgG) to Human SPHK / SPHK1
Human
ELISA, IHC, WB
Unconjugated
100µg  $355.00
Showing 1-25 of 81 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).