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| Catalog Number | Size | Price |
|---|---|---|
| LS-A3106-50 | 50 µg (1 mg/ml) | $440 |
IHC‑plus™ Polyclonal Rabbit anti‑Human SPHK / SPHK1 Antibody (N‑Terminus, IHC) LS‑A3106
IHC‑plus™ Polyclonal Rabbit anti‑Human SPHK / SPHK1 Antibody (N‑Terminus, IHC) LS‑A3106
Note: This antibody replaces LS-C42476
Antibody:
SPHK / SPHK1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Overview
Antibody:
SPHK / SPHK1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Specifications
Description
SPHK1 antibody LS-A3106 is an unconjugated rabbit polyclonal antibody to SPHK1 (SPHK) (N-Terminus) from human. It is reactive with human and monkey. Validated for IHC. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human SPHK / SPHK1
Synonyms
SPHK1 | SK1 | Sphingosine kinase 1 | SPHK | SK 1 | SPK 1 | Sphingosine kinase type 1 | SPK
Host
Rabbit
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Predicted
Bat, Dog, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 15 amino acid peptide from N-Terminus of human SPHK1. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%); Gorilla, Dog, Bat, Elephant, Panda, Rabbit (93%); Horse (87%); Mouse, Rat, Bovine (80%).
Epitope
N-Terminus
Specificity
Human SPHK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SPHK / SPHK1
Validation
Anti-SPHK1 antibody IHC of human adrenal, pheochromocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SPHK1 antibody IHC of human adrenal, pheochromocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
See More About...
LSBio Ratings
IHC-plus™ SPHK / SPHK1 Antibody (N-Terminus) for IHC LS-A3106 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Bone morphogenetic protein-2 enhances gonadotropin-independent follicular development via sphingosine kinase 1. Masami Ito, Osamu Yoshino, Yosuke Ono, Akemi Yamaki-Ushijima, Tomoko Tanaka, Tomoko Shima, Makoto Orisaka, Akira Iwase, Akitoshi Nakashima, Shigeru Saito. American journal of reproductive immunology (New York, N.Y. : 1989). 2021 May;85:e13374.
Customer Reviews (0)
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Species:
Human, Monkey, Mouse, Hamster
Applications:
Western blot, Immunoprecipitation, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin
Species:
Human, Mouse, Rat, Primate
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey, Mouse, Hamster
Applications:
IHC, IHC - Paraffin, ICC, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
