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Catalog Number Size Price
LS-C408278-20 20 µl $287 
LS-C408278-100 100 µl $431 
Rho Kinase / ROCK1 Antibody - Immunohistochemistry of paraffin-embedded human stomach using ROCK1 antibodyat dilution of 1:100 (40x lens).
Rho Kinase / ROCK1 Antibody - Immunohistochemistry of paraffin-embedded rat heart using ROCK1 antibodyat dilution of 1:100 (40x lens).
Rho Kinase / ROCK1 Antibody - Western blot analysis of extracts from normal (control) and ROCK1 knockout (KO) HeLa cells, using ROCK1 antibodyat 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 3s.
Rho Kinase / ROCK1 Antibody - Western blot analysis of extracts of various cell lines, using ROCK1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Rho Kinase / ROCK1 Antibody - Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug ROCK1 antibody. Western blot was performed from the immunoprecipitate using ROCK1 antibodyat a dilition of 1:1000.
Rho Kinase / ROCK1 Antibody - Immunohistochemistry of paraffin-embedded human stomach using ROCK1 antibodyat dilution of 1:100 (40x lens).
Rho Kinase / ROCK1 Antibody - Immunohistochemistry of paraffin-embedded rat heart using ROCK1 antibodyat dilution of 1:100 (40x lens).
Rho Kinase / ROCK1 Antibody - Western blot analysis of extracts from normal (control) and ROCK1 knockout (KO) HeLa cells, using ROCK1 antibodyat 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 3s.
Rho Kinase / ROCK1 Antibody - Western blot analysis of extracts of various cell lines, using ROCK1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Rho Kinase / ROCK1 Antibody - Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug ROCK1 antibody. Western blot was performed from the immunoprecipitate using ROCK1 antibodyat a dilition of 1:1000.
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Polyclonal Rabbit anti‑Human Rho Kinase / ROCK1 Antibody (IHC, WB) LS‑C408278

Polyclonal Rabbit anti‑Human Rho Kinase / ROCK1 Antibody (IHC, WB) LS‑C408278

Antibody:
Rho Kinase / ROCK1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$287
LS-C408278-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Rho Kinase / ROCK1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ROCK1 antibody LS-C408278 is an unconjugated rabbit polyclonal antibody to ROCK1 (Rho Kinase) from human. It is reactive with human, mouse and rat. Validated for IHC, IP and WB.
Target
Human Rho Kinase / ROCK1
Synonyms
ROCK1 | p160 Rho-associated kinase | p160ROCK | p160 ROCK-1 | p160-ROCK | p160RhoK | Rho-associated kinase | RockI | Rok | Rok alpha | Rho-alpha kinase | p160 rho-kinase | Rho kinase | ROCK-I | Rokalpha
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1). YSNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLN
Specificity
Human Rho Kinase / ROCK1
Applications
  • IHC (1:50 - 1:100)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 158kDa, while the observed MW by Western blot was 158kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Rho Kinase / ROCK1
Q13464 NM_005406 NP_005397.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/19/2024