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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IHCPlus™ Rho Kinase / ROCK1 Antibody Polyclonal ELISA,ICC,IF,IHC,WB LS‑B7100

Catalog Number Size Price
LS-B7100-0.05 0.05 mg (1 mg/ml) $425 
Anti-ROCK1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunocytochemistry of ROCK1 in 293 cells with ROCK1 antibody at 10 ug/ml.
Immunofluorescence of ROCK1 in 293 cells with ROCK1 antibody at 20 ug/ml.
Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Anti-ROCK1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunocytochemistry of ROCK1 in 293 cells with ROCK1 antibody at 10 ug/ml.
Immunofluorescence of ROCK1 in 293 cells with ROCK1 antibody at 20 ug/ml.
Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
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IHCPlus™ Rho Kinase / ROCK1 Antibody Polyclonal ELISA,ICC,IF,IHC,WB LS‑B7100

Note: This antibody replaces LS-C144365
Antibody:
Rabbit Polyclonal to Human Rho Kinase / ROCK1
Application:
IHC, IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B7100-0.05
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human Rho Kinase / ROCK1
Application:
IHC, IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ROCK1 antibody LS-B7100 is an unconjugated rabbit polyclonal antibody to ROCK1 (Rho Kinase ) from human, mouse and rat. Validated for ELISA, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Rho Kinase / ROCK1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
a 15 amino acid synthetic peptide near the N-terminus of human ROCK1. The immunogen is located within the first 50 amino acids of ROCK1.
Blocking Peptide
LS-E31111 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-B7100. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Specificity
ROCK1 antibody is predicted to not cross-react with other ROCK protein family members.
Usage
ROCK1 antibody can be used for detection of ROCK1 by Western blot at 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Rho Kinase / ROCK1
Q13464 NM_005406 NP_005397.1

Validation

Anti-ROCK1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-ROCK1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ROCK1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 8/20/2019
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