ROCK1 antibody LS-C511 is an unconjugated mouse monoclonal antibody to ROCK1 (Rho Kinase) from human, mouse, rat and other species. Validated for WB.
Human Rho Kinase / ROCK1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse
(tested or 100% immunogen sequence identity)
Rabbit (at least 90% immunogen sequence identity)
Protein G purified
- Western blot (1 - 2 µg/ml)
This antibody was generated by immunizing mice with a synthetic peptide sequence corresponding to residues 1114-1126 GNLPESRIEGWLS of human ROCK-1. Immunogen peptide sequence is adjacent to the caspase-3 cleavage site of ROCK-1. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Bovine, Dog, Bat, Horse, Opossum, Platypus (100%); Rabbit (92%); Chicken (85%).
This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for detection of apoptosis.
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.