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RFP / Red Fluorescent Protein Antibody (N‑Terminus, clone RF5R) LS‑C153602

Catalog Number Size Price
LS-C153602-100 100 µg (1 mg/ml) $305 
Antibody

RFP / Red Fluorescent Protein Antibody (N‑Terminus, clone RF5R) LS‑C153602

Antibody:
Mouse Monoclonal to Sea anemone RFP / Red Fluorescent Protein
Application:
WB, IP, ELISA
Reactivity:
Sea anemone
Format:
Unconjugated, Unmodified
Price
Catalog Number
$305 
LS-C153602-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Sea anemone RFP / Red Fluorescent Protein
Application:
WB, IP, ELISA
Reactivity:
Sea anemone
Format:
Unconjugated, Unmodified

Specifications

Description
Red Fluorescent Protein antibody LS-C153602 is an unconjugated mouse monoclonal antibody to sea anemone Red Fluorescent Protein (RFP ). Validated for ELISA, IP and WB.
Target
Sea anemone RFP / Red Fluorescent Protein
Host
Mouse
Reactivity
Sea anemone (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
RF5R
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • ELISA
Immunogen
RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates.
Epitope
N-Terminus
Specificity
Reacts with native and denatured forms of RFP and its variants: tag-RFP, turbo-RFP, DeRed, mCherry, mOrange, etc.
Presentation
10 mM PBS, pH 7.2
Storage
Long term store at -20°C in undiluted aliquots. Short term store at 4°C with 0.1% sodium azide. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Featured Products

Anti-RFP Antibody - Western Blot. Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kD band. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2500 for 1 h at RT followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Goat-a-Rabbit IgG [H&L] MX (. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Species: Discosoma
Applications: IHC, Western blot, ELISA
Product
Species: Discosoma
Applications: Western blot
Product
Species: Discosoma
Applications: Western blot, Immunoprecipitation, ELISA
Immunocytochemistry Image
Species:
Applications: ICC, Western blot
Product
Species: Discosoma
Applications: Immunofluorescence, Western blot, ELISA
Western blot of Anti-RFP Antibody. 0.1 ug of RFP was loaded on a 4-20% gel and transferred to nitrocellulose membrane. Anti-RFP Antibody was added at 1.0 g/mL at RT for 2 hours. IRDye800 anti-Chicken (p/n 603-132-126) was added at 1:20000 at RT for 45 minutes.
Species: Discosoma
Applications: Western blot, ELISA

Requested From: United States
Date Requested: 8/22/2019
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