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Catalog Number Size Price
LS-C744819-25 25 µl (1 mg/ml) $275 
RFP / Red Fluorescent Protein Antibody - Western Blot of Mouse Anti-RFP antibody. Lane 1: YFP protein. Lane 2: Prestained Molecular Weight Marker. Lane 3: Reduced RFP control Protein. Lane 4: Reduced mCherry. Lane 5: GFP protein. Lane 6: Non-Reduced RFP control Protein. Lane 7: Non-Reduced mCherry. Load: 300ng per lane. Primary antibody: RFP antibody at 1:2000 in MB-070 for 3 hours at RT. Secondary antibody: HRP anti-Mouse secondary antibody at 1:10,000 in MB-070 for 60 min at RT. Substrate: TMBM-100 for 20 min. Predicted/Observed size: ~27 kDa.
RFP / Red Fluorescent Protein Antibody - ELISA results of purified Mouse anti-RFP Monoclonal Antibody tested against RFP Each well was coated in duplicate with 1.0 µg of the antigen. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, anti-Mouse IgG Antibody Peroxidase Conjugated Aecondary and TMB ELISA Peroxidase Substrate
RFP / Red Fluorescent Protein Antibody - Western Blot of Mouse Anti-RFP antibody. Lane 1: YFP protein. Lane 2: Prestained Molecular Weight Marker. Lane 3: Reduced RFP control Protein. Lane 4: Reduced mCherry. Lane 5: GFP protein. Lane 6: Non-Reduced RFP control Protein. Lane 7: Non-Reduced mCherry. Load: 300ng per lane. Primary antibody: RFP antibody at 1:2000 in MB-070 for 3 hours at RT. Secondary antibody: HRP anti-Mouse secondary antibody at 1:10,000 in MB-070 for 60 min at RT. Substrate: TMBM-100 for 20 min. Predicted/Observed size: ~27 kDa.
RFP / Red Fluorescent Protein Antibody - ELISA results of purified Mouse anti-RFP Monoclonal Antibody tested against RFP Each well was coated in duplicate with 1.0 µg of the antigen. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, anti-Mouse IgG Antibody Peroxidase Conjugated Aecondary and TMB ELISA Peroxidase Substrate
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Monoclonal Mouse RFP / Red Fluorescent Protein Antibody (clone 8E5.G7, IF, WB) LS‑C744819

Monoclonal Mouse RFP / Red Fluorescent Protein Antibody (clone 8E5.G7, IF, WB) LS‑C744819

Antibody:
RFP / Red Fluorescent Protein Mouse Monoclonal (8E5.G7) Antibody
Application:
IF, WB, IP, Flo, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C744819-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
RFP / Red Fluorescent Protein Mouse Monoclonal (8E5.G7) Antibody
Application:
IF, WB, IP, Flo, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
Red Fluorescent Protein antibody LS-C744819 is an unconjugated mouse monoclonal antibody to Red Fluorescent Protein (RFP). Validated for ELISA, Flow, IF, IP and WB.
Target
RFP / Red Fluorescent Protein
Host
Mouse
Clonality
IgG2a,k Monoclonal
Clone
8E5.G7
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Anti-RFP monoclonal antibody is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Applications
  • Immunofluorescence
  • Western blot (1:1000 - 1:10000)
  • Immunoprecipitation
  • Flow Cytometry
  • ELISA (1:75000 - 1:150000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/10/2021