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RFP / Red Fluorescent Protein Antibody (HRP) LS‑C154272

Catalog Number Size Price
LS-C154272-100 100 µg (1 mg/ml) $425 
Antibody

RFP / Red Fluorescent Protein Antibody (HRP) LS‑C154272

Antibody:
Rabbit Polyclonal to Discosoma RFP / Red Fluorescent Protein
Application:
IHC, WB, ELISA
Reactivity:
Discosoma
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$425 
LS-C154272-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Discosoma RFP / Red Fluorescent Protein
Application:
IHC, WB, ELISA
Reactivity:
Discosoma
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
Red Fluorescent Protein antibody LS-C154272 is an HRP-conjugated rabbit polyclonal antibody to discosoma Red Fluorescent Protein (RFP ). Validated for ELISA, IHC and WB.
Target
Discosoma RFP / Red Fluorescent Protein
Host
Rabbit
Reactivity
Discosoma (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:500 - 1:2000)
  • Western blot (1:2000 - 1:5000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Specificity
Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal.
Usage
Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% Gentamicin sulfate
Reconstitution
Reconstitute in deionized water
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Featured Products

Anti-RFP Antibody - Western Blot. Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kD band. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2500 for 1 h at RT followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Goat-a-Rabbit IgG [H&L] MX (. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Species: Discosoma
Applications: IHC, Western blot, ELISA
Product
Species: Discosoma
Applications: Western blot
Product
Species: Discosoma
Applications: Western blot, Immunoprecipitation, ELISA
Immunocytochemistry Image
Species:
Applications: ICC, Western blot
Product
Species: Discosoma
Applications: Immunofluorescence, Western blot, ELISA
Western blot of Anti-RFP Antibody. 0.1 ug of RFP was loaded on a 4-20% gel and transferred to nitrocellulose membrane. Anti-RFP Antibody was added at 1.0 g/mL at RT for 2 hours. IRDye800 anti-Chicken (p/n 603-132-126) was added at 1:20000 at RT for 45 minutes.
Species: Discosoma
Applications: Western blot, ELISA

Requested From: United States
Date Requested: 8/25/2019
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