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Catalog Number Size Price
LS-C209430-100 100 µg (1 mg/ml) $567 
RFP / Red Fluorescent Protein Antibody - Immunofluorescence Microscopy of Chicken Anti-RFP antibody. Tissue: DsRed transgenic mouse retina. Fixation: 4% PFA.3% BSA, 0.3% Triton Primary antibody: RFP-biotin antibody at 1:100 for 12 h at 4°C. Secondary antibody: Alexa488 secondary antibody at 1:10,000 for 4 hours at RT. Localization: RFP is nuclear and occasionally cytoplasmic. Staining: labeled in red.
RFP / Red Fluorescent Protein Antibody - Western blot of Anti-RFP Antibody. 0.1 ug of RFP was loaded on a 4-20% gel and transferred to nitrocellulose membrane. Anti-RFP Antibody was added at 1.0 ug/mL at RT for 2 hours. IRDye800 anti-Chicken (p/n 603-132-126) was added at 1:20,000 at RT for 45 minutes. This image was taken for the unconjugated form of this product. Other forms have not been tested.
RFP / Red Fluorescent Protein Antibody - Immunofluorescence Microscopy of Chicken Anti-RFP antibody. Tissue: DsRed transgenic mouse retina. Fixation: 4% PFA.3% BSA, 0.3% Triton Primary antibody: RFP-biotin antibody at 1:100 for 12 h at 4°C. Secondary antibody: Alexa488 secondary antibody at 1:10,000 for 4 hours at RT. Localization: RFP is nuclear and occasionally cytoplasmic. Staining: labeled in red.
RFP / Red Fluorescent Protein Antibody - Western blot of Anti-RFP Antibody. 0.1 ug of RFP was loaded on a 4-20% gel and transferred to nitrocellulose membrane. Anti-RFP Antibody was added at 1.0 ug/mL at RT for 2 hours. IRDye800 anti-Chicken (p/n 603-132-126) was added at 1:20,000 at RT for 45 minutes. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Polyclonal Chicken anti‑Discosoma RFP / Red Fluorescent Protein Antibody (Biotin, IF, WB) LS‑C209430

Polyclonal Chicken anti‑Discosoma RFP / Red Fluorescent Protein Antibody (Biotin, IF, WB) LS‑C209430

Antibody:
RFP / Red Fluorescent Protein Chicken anti-Discosoma Polyclonal (Biotin) Antibody
Application:
IF, WB, ELISA
Reactivity:
Discosoma
Format:
Biotin, Unmodified
Price
Catalog Number
$567
LS-C209430-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
RFP / Red Fluorescent Protein Chicken anti-Discosoma Polyclonal (Biotin) Antibody
Application:
IF, WB, ELISA
Reactivity:
Discosoma
Format:
Biotin, Unmodified

Specifications

Description
Red Fluorescent Protein antibody LS-C209430 is a biotin-conjugated chicken polyclonal antibody to discosoma Red Fluorescent Protein (RFP). Validated for ELISA, IF and WB.
Target
Discosoma RFP / Red Fluorescent Protein
Host
Chicken
Reactivity
Discosoma (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Filtration, delipidation, salt fractionation and dialysis.
Modifications
Unmodified
Immunogen
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000 - 1:3000)
  • ELISA (1:10000)
Usage
Anti-RFP is designed to detect recombinant RFP. This antibody can be used to detect RFP by immunoblotting. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/30/2024