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| Catalog Number | Size | Price |
|---|---|---|
| LS-C35197-50 | 50 µg | $947 |
Polyclonal Rabbit anti‑Human PTK6 / BRK Antibody (N‑Terminus, WB) LS‑C35197
Polyclonal Rabbit anti‑Human PTK6 / BRK Antibody (N‑Terminus, WB) LS‑C35197
Antibody:
PTK6 / BRK Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
PTK6 / BRK Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
BRK antibody LS-C35197 is an unconjugated rabbit polyclonal antibody to human BRK (PTK6) (N-Terminus). Validated for WB.
Target
Human PTK6 / BRK
Synonyms
PTK6 | BRK | SIK | Src-related intestinal kinase | Tks | Tyrosine-protein kinase BRK | Breast tumor kinase | Protein-tyrosine kinase 6 | Protein-tyrosine kinase BRK | PTK6 protein tyrosine kinase 6
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to the N-Terminus of human Brk.
Epitope
N-Terminus
Specificity
Recognizes Brk (breast tumor kinase, PTK6) at approximately 52 kD. It can also recognize the 73 kD ELF-related protein that shares 70% homology with the immunizing peptide.
Applications
- Western blot (1:500)
Usage
Western Blot: 1:500 dilution detected endogenous Brk in SKBR3 cell lysate. Lysate was resolved by electrophoresis, transferred to PVDF, and probed for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Band observed at ~50kD indicates Brk. An upper band is thought to be the 73kD ELF-related protein that shares 70% homology with the immunizing peptide. Optimal dilutions to be determined by the researcher.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Restrictions
For research use only. Intended for use by laboratory professionals.
About PTK6 / BRK
Publications (0)
Customer Reviews (0)
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Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Reactivity:
Monkey
Range:
0.78-50 ng/ml
Reactivity:
Mouse
Range:
0.625-40 ng/ml
Species:
Mouse, Human, Chimpanzee, Gibbon, Monkey, Rat, Bovine, Pig
Applications:
Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
Date Requested: 4/28/2024
