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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PTK6 / BRK

protein tyrosine kinase 6

Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways.Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins.

Gene Name: protein tyrosine kinase 6
Family/Subfamily: Protein Kinase , Src
Synonyms: PTK6, BRK, SIK, Src-related intestinal kinase, Tks, Tyrosine-protein kinase BRK, Breast tumor kinase, Protein-tyrosine kinase 6, Protein-tyrosine kinase BRK, PTK6 protein tyrosine kinase 6
Target Sequences: NM_005975 NP_005966.1 Q13882

Publications (1)

1
Low expression of PTK6/Brk predicts poor prognosis in patients with laryngeal squamous cell carcinoma. Liu XK, Zhang XR, Zhong Q, Li MZ, Liu ZM, Lin ZR, Wu D, Zeng MS. Journal of translational medicine. 2013 11:59. [PubMed:23497344] [PMC:PMC3599503] Related Antibodies: LS-C99386.

Filtered By: None
Target
PTK6 / BRK (57)
Reactivity
Human (55)
Mouse (18)
Rat (4)
Horse (4)
Application
IHC (24)
IHC-P (2)
WB (47)
ELISA (29)
ICC (3)
IF (10)
IP (1)
Peptide-ELISA (3)
Host
rabbit (53)
mouse (4)
Isotype
IgG (47)
IgG2b (2)
IgG2b,k (1)
Clonality
monoclonal mc (3)
polyclonal pc (54)
Clone
2F11 (1)
2H12B8 (2)
Format
AP Conjugated (2)
APC (1)
APC Conjugated (3)
Azide-free (2)
Biotin Conjugated (6)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (6)
HRP Conjugated (5)
PE Conjugated (3)
Unconjugated (30)
Epitope
aa191-445 (10)
aa1-28 (8)
aa11-41 (8)
aa287-336 (4)
390-470 aa (2)
10-90 aa, N-terminal (1)
N-Terminus (1)
aa10-59 (1)
aa402-451 (1)
aa508 (1)
pTyr342 (1)
pTyr447 (1)
Publications
No (56)
Yes (1)
Showing 1-25 of 57 results

PTK6 / BRK Antibody (aa11-41) LS-C99386
Publications (1)
Publications (1)
Rabbit Polyclonal to Human PTK6 / BRK
Human
IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-hPTK6-E26 antibody in A375 cell line lysate (35 ug/lane). hPTK6-E26(arrow) was detected using the purified antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-hPTK6-E26 antibody in A375 cell line lysate (35 ug/lane). hPTK6-E26(arrow) was detected using the purified antibody.

PTK6 / BRK Antibody (phospho-Tyr342) LS-C152797
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
WB
Unconjugated
100µl 0.5mg/ml  $415.00
WB: IGF-1 stimulated lysates from 293T cells transfected with Brk (Lane1) and mutant Brk (Y342A) (lane2) were resolved by electrophoresis, transferred to PVDF, and probed with anti-phospho Brk(Y342) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Brk(Y342).
WB: IGF-1 stimulated lysates from 293T cells transfected with Brk (Lane1) and mutant Brk (Y342A) (lane2) were resolved by electrophoresis, transferred to PVDF, and probed with anti-phospho Brk(Y342) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Brk(Y342).

PTK6 / BRK Antibody LS-C668173
Rabbit Polyclonal to Human PTK6 / BRK
Human
ICC IF IHC IHC-P WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $275.00
Immunohistochemistry - Paraffin Image
Immunofluorescence Image
Immunohistochemistry - Paraffin Image
Immunofluorescence Image

PTK6 / BRK Antibody (10-90 aa, N-terminal) LS-C382320
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Mouse, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00

PTK6 / BRK Antibody LS-C660502
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA IF IHC IP WB
Unconjugated
100µg  $405.00

PTK6 / BRK Antibody LS-C349190
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Mouse, Rat, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded human gastric cancer tissue.
Immunofluorescence analysis of A549 cells.
Immunohistochemistry of paraffin-embedded human gastric cancer tissue.
Immunofluorescence analysis of A549 cells.

PTK6 / BRK Antibody (aa11-41) LS-C326962
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA IHC WB
Unconjugated
200µl 0.25mg/ml  $530.00

PTK6 / BRK Antibody (aa11-41, Biotin) LS-C275667
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA IHC WB
Biotin Conjugated
200µl  $680.00

PTK6 / BRK Antibody (aa11-41, FITC) LS-C275668
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA IHC WB
FITC Conjugated
200µl  $680.00

PTK6 / BRK Antibody (aa11-41, APC) LS-C275665
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA IHC WB
APC Conjugated
200µl  $680.00

PTK6 / BRK Antibody (aa11-41, AP) LS-C275666
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA IHC WB
AP Conjugated
200µl  $680.00

PTK6 / BRK Antibody (aa11-41, PE) LS-C275672
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA IHC WB
PE Conjugated
200µl  $680.00

PTK6 / BRK Antibody (aa11-41, Azide-free, HRP) LS-C275669
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA IHC WB
HRP Conjugated, Azide-free
200µl  $680.00

PTK6 / BRK Antibody (390-470 aa) LS-C381130
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA IF WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Phospho-Brk (Y447) antibody
Western blot of Phospho-Brk (Y447) antibody

PTK6 / BRK Antibody LS-C500738
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA IF WB
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunofluorescent analysis of MCF-7 cells using PTK6 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Positive WB detected in:MCF-7 whole cell lysate,Hela whole cell lysate;All lanes:PTK6 antibody at 2.4?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 52,15 KDa;Observed band size: 52 KDa;
Immunofluorescent analysis of MCF-7 cells using PTK6 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Positive WB detected in:MCF-7 whole cell lysate,Hela whole cell lysate;All lanes:PTK6 antibody at 2.4?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 52,15 KDa;Observed band size: 52 KDa;

PTK6 / BRK Antibody (phospho-Tyr447) LS-C198699
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
IF Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunofluorescence analysis of NIH/3T3 cells, using Breast Tumor Kinase (Phospho-Tyr447) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using Breast Tumor Kinase (Phospho-Tyr447) Antibody. The lane on the right is blocked with the phospho peptide.
Immunofluorescence analysis of NIH/3T3 cells, using Breast Tumor Kinase (Phospho-Tyr447) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using Breast Tumor Kinase (Phospho-Tyr447) Antibody. The lane on the right is blocked with the phospho peptide.

PTK6 / BRK Antibody (390-470 aa) LS-C382321
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA IF
Unconjugated
100µl 1mg/ml  $265.00

PTK6 / BRK Antibody (aa1-28) LS-C324407
Rabbit Polyclonal (IgG) to Mouse PTK6 / BRK
Mouse, Human
ELISA WB
Unconjugated
200µl  $530.00

PTK6 / BRK Antibody LS-C401458
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Mouse, Human
ELISA IHC
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human gastric cancer using PTK6 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human gastric cancer using PTK6 Polyclonal Antibody at dilution of 1:40.

PTK6 / BRK Antibody LS-C412960
Rabbit Polyclonal to Human PTK6 / BRK
Mouse, Rat, Human
IHC WB
Unconjugated
100µl  $385.00

PTK6 / BRK Antibody (aa191-445) LS-C373258
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $530.00; 10ml 0.5mg/ml  $2,100.00
Western blot of PTK6 / BRK antibody.
Western blot of PTK6 / BRK antibody.

PTK6 / BRK Antibody LS-C332689
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
IHC WB
Unconjugated
50µl  $305.00
Western blot analysis of extracts of MCF7 cell line, using PTK6 antibody.
Western blot analysis of extracts of MCF7 cell line, using PTK6 antibody.

PTK6 / BRK Antibody LS-C404751
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Mouse, Human
ELISA IHC
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human cervical cancer using PTK6 Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of Human cervical cancer using PTK6 Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of Human cervical cancer using PTK6 Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of Human cervical cancer using PTK6 Polyclonal Antibody at dilution of 1:30.

PTK6 / BRK Antibody (clone 2H12B8) LS-C82169
Mouse Monoclonal [clone 2H12B8] (IgG2b) to Human PTK6 / BRK
Human
ELISA WB
Unconjugated
100µl  $475.00
Western blot of PTK6 monoclonal antibody against truncated PTK6 recombinant protein and MCF-7 cell lysate.
Western blot of PTK6 monoclonal antibody against truncated PTK6 recombinant protein and MCF-7 cell lysate.

PTK6 / BRK Antibody (clone 2H12B8) LS-C47211
Mouse Monoclonal [clone 2H12B8] (IgG2b) to Human PTK6 / BRK
Human
ELISA WB
Unconjugated
100µl  $365.00
Western blot Image
Western blot Image
Showing 1-25 of 57 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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