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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PTK6 / BRK Antibody (aa11‑41) LS‑C99386

Catalog Number Size Price
LS-C99386-400 400 µl $325 
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-hPTK6-E26 antibody in A375 cell line lysate (35 ug/lane). hPTK6-E26(arrow) was detected using the purified antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-hPTK6-E26 antibody in A375 cell line lysate (35 ug/lane). hPTK6-E26(arrow) was detected using the purified antibody.
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PTK6 / BRK Antibody (aa11‑41) LS‑C99386

Antibody:
Rabbit Polyclonal to Human PTK6 / BRK
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C99386-400
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human PTK6 / BRK
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BRK antibody LS-C99386 is an unconjugated rabbit polyclonal antibody to human BRK (PTK6 ). Validated for IHC and WB. Cited in 1 publication.
Target
Human PTK6 / BRK
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9218 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C99386. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa11-41
Specificity
This BRK (PTK6) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-41 amino acids from the N-terminal region of human BRK (PTK6).
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PTK6 / BRK
Q13882 NM_005975 NP_005966.1

Publications (1)

Low expression of PTK6/Brk predicts poor prognosis in patients with laryngeal squamous cell carcinoma. Liu XK, Zhang XR, Zhong Q, Li MZ, Liu ZM, Lin ZR, Wu D, Zeng MS. Journal of translational medicine. 2013 11:59.

Reviews (0)

Featured Products

WB: IGF-1 stimulated lysates from 293T cells transfected with Brk (Lane1) and mutant Brk (Y342A) (lane2) were resolved by electrophoresis, transferred to PVDF, and probed with anti-phospho Brk(Y342) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Brk(Y342).
Species: Human
Applications: Western blot
Immunofluorescence analysis of NIH/3T3 cells, using Breast Tumor Kinase (Phospho-Tyr447) Antibody. The picture on the right is blocked with the phospho peptide.
Species: Human
Applications: Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Reactivity: Human
Range: 0.312-20 ng/ml
Reactivity: Human
Range: 0.156-10 ng/ml

Requested From: United States
Date Requested: 9/23/2019