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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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PTK6 / BRK

protein tyrosine kinase 6

Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways.Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins.

Gene Name: protein tyrosine kinase 6
Family/Subfamily: Protein Kinase , Src
Synonyms: PTK6, BRK, SIK, Src-related intestinal kinase, Tks, Tyrosine-protein kinase BRK, Breast tumor kinase, Protein-tyrosine kinase 6, Protein-tyrosine kinase BRK, PTK6 protein tyrosine kinase 6
Target Sequences: NM_005975 NP_005966.1 Q13882

Publications (1)

1
Low expression of PTK6/Brk predicts poor prognosis in patients with laryngeal squamous cell carcinoma. Liu XK, Zhang XR, Zhong Q, Li MZ, Liu ZM, Lin ZR, Wu D, Zeng MS. Journal of translational medicine. 2013 11:59. [PubMed:23497344] [PMC:PMC3599503] Related Antibodies: LS-C99386.


PTK6 / BRK Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Rabbit Polyclonal to Human PTK6 / BRK
Human
IHC, IHC-P, WB
Unconjugated
400µl   $325.00
PTK6 / BRK Antibody - WB: IGF-1 stimulated lysates from 293T cells transfected with Brk (Lane1) and mutant Brk (Y342A) (lane2) were resolved by electrophoresis, transferred to PVDF, and probed with anti-phospho Brk(Y342) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Brk(Y342).
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
WB
Unconjugated
100µl   0.5mg/ml   $415.00
PTK6 / BRK Antibody - Immunofluorescence analysis of NIH/3T3 cells, using Breast Tumor Kinase (Phospho-Tyr447) Antibody. The picture on the right is blocked with the phospho peptide.
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
IF, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
PTK6 / BRK Antibody
Select
Rabbit Polyclonal to Human PTK6 / BRK
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $275.00
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA, IF, IHC, IP, WB
Unconjugated
100µg   $405.00
PTK6 / BRK Antibody - Immunohistochemistry of paraffin-embedded human gastric cancer tissue.
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Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20µl   $215.00; 50µl   $235.00; 100µl   $315.00; 200µl   $425.00
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
60µl   1mg/ml   $235.00; 120µl   1mg/ml   $295.00; 200µl   1mg/ml   $395.00
PTK6 / BRK Antibody - Immunofluorescent analysis of BRK (pY447) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Rabbit Polyclonal to Human PTK6 / BRK
Rat, Human
ICC, IF, WB
Unconjugated
30µl   1mg/ml   $215.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $325.00
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA, IHC, WB
Unconjugated
200µl   $590.00
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA, IHC, WB
Biotin Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA, IHC, WB
FITC Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA, IHC, WB
APC Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA, IHC, WB
AP Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA, IHC, WB
PE Conjugated
200µl   $740.00
PTK6 / BRK Antibody - Western blot of Phospho-Brk (Y447) antibody
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Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA, IF, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
PTK6 / BRK Antibody - Immunofluorescent analysis of MCF-7 cells using PTK6 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA, IF, WB
Unconjugated
50µg   $285.00; 100µg   $385.00
PTK6 / BRK Antibody - P-peptide - + Immunofluorescence analysis of NIH/3T3 cells, using Breast Tumor Kinase (Phospho-Tyr447) antibody.
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Mouse, Human
ELISA, IF, WB
Unconjugated
100µg   $305.00
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA, IF
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
PTK6 / BRK Antibody - Peptide - + Immunofluorescence analysis of 3T3 cells, using Breast Tumor Kinase antibody.
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
ELISA, IF
Unconjugated
100µg   $305.00
Select
Rabbit Polyclonal to Human PTK6 / BRK
Mouse, Rat, Human
IHC, WB
Unconjugated
100µl   $385.00
PTK6 / BRK Antibody - Immunohistochemistry of paraffin-embedded Human gastric cancer using PTK6 Polyclonal Antibody at dilution of 1:40.
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Mouse, Human
ELISA, IHC
Unconjugated
20µl   0.3mg/ml   $205.00; 60µl   0.3mg/ml   $235.00; 120µl   0.3mg/ml   $295.00; 200µl   0.3mg/ml   $395.00
Select
Rabbit Polyclonal (IgG) to Mouse PTK6 / BRK
Mouse, Human
ELISA, WB
Unconjugated
200µl   $590.00
PTK6 / BRK Antibody - Western blot analysis of extracts of MCF7 cell line, using PTK6 antibody.
Select
Rabbit Polyclonal (IgG) to Human PTK6 / BRK
Human
IHC, WB
Unconjugated
100µl   $295.00; 200µl   $385.00
Viewing 1-25 of 67 products



If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).