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Antibodies > PTK6 / BRK > LS-C761123
 
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Catalog Number Size Price
LS-C761123-30 30 µl (1 mg/ml) $263 
LS-C761123-100 100 µl (1 mg/ml) $315 
LS-C761123-200 200 µl (1 mg/ml) $379 
PTK6 / BRK Antibody - Immunofluorescent analysis of BRK (pY447) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
PTK6 / BRK Antibody - Western blot analysis of BRK (pY447) expression in HEK293T (A), rat kidney (B), rat lung (C) whole cell lysates.
PTK6 / BRK Antibody - Immunofluorescent analysis of BRK (pY447) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
PTK6 / BRK Antibody - Western blot analysis of BRK (pY447) expression in HEK293T (A), rat kidney (B), rat lung (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human PTK6 / BRK Antibody (IF, WB) LS‑C761123

Polyclonal Rabbit anti‑Human PTK6 / BRK Antibody (IF, WB) LS‑C761123

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Antibody:
PTK6 / BRK Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$263
LS-C761123-30
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PTK6 / BRK Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
BRK antibody LS-C761123 is an unconjugated rabbit polyclonal antibody to BRK (PTK6) from human. It is reactive with human and rat. Validated for ICC, IF and WB.
Target
Human PTK6 / BRK
Synonyms
PTK6 | BRK | SIK | Src-related intestinal kinase | Tks | Tyrosine-protein kinase BRK | Breast tumor kinase | Protein-tyrosine kinase 6 | Protein-tyrosine kinase BRK | PTK6 protein tyrosine kinase 6
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human BRK. The exact sequence is proprietary.
Specificity
Recognizes endogenous levels of BRK (pY447) protein.
Applications
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Usage
Applications should be user optimized.
Presentation
0.42% Potassium Phosphate, pH 7.3, 0.87% NaCl, 0.01% Sodium Azide, 30% Glycerol
Storage
Upon delivery, aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PTK6 / BRK
Q13882 NM_005975 NP_005966.1

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Featured Products

PTK6 / BRK Antibody - WB: IGF-1 stimulated lysates from 293T cells transfected with Brk (Lane1) and mutant Brk (Y342A) (lane2) were resolved by electrophoresis, transferred to PVDF, and probed with anti-phospho Brk(Y342) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Brk(Y342).
PTK6 / BRK Antibody (phospho‑Tyr342) LS‑C152797
Species: Human
Applications: Western blot
PTK6 / BRK Antibody - Immunofluorescence analysis of NIH/3T3 cells, using Breast Tumor Kinase (Phospho-Tyr447) Antibody. The picture on the right is blocked with the phospho peptide.
PTK6 / BRK Antibody (phospho‑Tyr447) LS‑C198699
Species: Human
Applications: Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
PTK6 / BRK Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
PTK6 / BRK Antibody (aa11‑41) LS‑C99386
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
CLDN18 / Claudin 18 Antibody - Human Stomach: Formalin-Fixed, Paraffin-Embedded (FFPE)
PathPlus™ CLDN18 / Claudin 18 Antibody LS‑B16145
Species: Human
Applications: IHC, IHC - Paraffin
RBP4 Antibody - Knockout Varification: Lane 1: Wild-type HepG2 cell lysate; Lane 2: RBP4 knockout HepG2 cell lysate; Predicted MW: 21kDa Observed MW: 21kDa Primary Ab: 1µg/ml Rabbit Anti-Bovine RBP4 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
RBP4 Antibody (aa1‑183) LS‑C296249
Species: Cow, Human, Mouse, Bovine, Pig
Applications: Western blot
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ITGA7 / Integrin Alpha 7 Antibody (Biotin) LS‑C680690
Species: Human
Applications: ELISA
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 9/18/2024
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