LSBio logo with text
Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > PTK6 / BRK > LS-C761123
 
Print Page  
Catalog Number Size Price
LS-C761123-30 30 µl (1 mg/ml) $263 
LS-C761123-100 100 µl (1 mg/ml) $315 
LS-C761123-200 200 µl (1 mg/ml) $379 
PTK6 / BRK Antibody - Immunofluorescent analysis of BRK (pY447) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
PTK6 / BRK Antibody - Western blot analysis of BRK (pY447) expression in HEK293T (A), rat kidney (B), rat lung (C) whole cell lysates.
PTK6 / BRK Antibody - Immunofluorescent analysis of BRK (pY447) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
PTK6 / BRK Antibody - Western blot analysis of BRK (pY447) expression in HEK293T (A), rat kidney (B), rat lung (C) whole cell lysates.
1 of 2
2 of 2

Polyclonal Rabbit anti‑Human PTK6 / BRK Antibody (IF, WB) LS‑C761123

Polyclonal Rabbit anti‑Human PTK6 / BRK Antibody (IF, WB) LS‑C761123

Submit Review LSBio Guarantee
Antibody:
PTK6 / BRK Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$263
LS-C761123-30
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PTK6 / BRK Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
BRK antibody LS-C761123 is an unconjugated rabbit polyclonal antibody to BRK (PTK6) from human. It is reactive with human and rat. Validated for ICC, IF and WB.
Target
Human PTK6 / BRK
Synonyms
PTK6 | BRK | SIK | Src-related intestinal kinase | Tks | Tyrosine-protein kinase BRK | Breast tumor kinase | Protein-tyrosine kinase 6 | Protein-tyrosine kinase BRK | PTK6 protein tyrosine kinase 6
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human BRK. The exact sequence is proprietary.
Specificity
Recognizes endogenous levels of BRK (pY447) protein.
Applications
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Usage
Applications should be user optimized.
Presentation
0.42% Potassium Phosphate, pH 7.3, 0.87% NaCl, 0.01% Sodium Azide, 30% Glycerol
Storage
Upon delivery, aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PTK6 / BRK
Q13882 NM_005975 NP_005966.1

Publications (0)

Submit a Publication for LS-C761123

Customer Reviews (0)

Submit a review for LS-C761123

Featured Products

PTK6 / BRK Antibody - WB: IGF-1 stimulated lysates from 293T cells transfected with Brk (Lane1) and mutant Brk (Y342A) (lane2) were resolved by electrophoresis, transferred to PVDF, and probed with anti-phospho Brk(Y342) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Brk(Y342).
PTK6 / BRK Antibody (phospho‑Tyr342) LS‑C152797
Species: Human
Applications: Western blot
PTK6 / BRK Antibody - Immunofluorescence analysis of NIH/3T3 cells, using Breast Tumor Kinase (Phospho-Tyr447) Antibody. The picture on the right is blocked with the phospho peptide.
PTK6 / BRK Antibody (phospho‑Tyr447) LS‑C198699
Species: Human
Applications: Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
PTK6 / BRK Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
PTK6 / BRK Antibody (aa11‑41) LS‑C99386
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
MOG ELISA Kit
Mouse MOG (Sandwich ELISA) ELISA Kit - LS-F34797
Reactivity: Mouse
Range: 0.156-10 ng/ml
SCN1A / Nav1.1 Antibody - Anti-SCN1A Antibody (Center) at 1:2000 dilution + U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 229 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
SCN1A / Nav1.1 Antibody (Internal) LS‑C757479
Species: Human
Applications: Western blot, Flow Cytometry, ELISA
Serotonin ELISA Kit
Mouse Serotonin (Competitive EIA) ELISA Kit - LS-F28406
Reactivity: Mouse
Range: 5-100 ng/ml
See all PTK6 / BRK Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 9/19/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2024 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com