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Catalog Number Size Price
LS-C800996-100 100 µl (1 mg/ml) $369 
LS-C800996-200 200 µl (1 mg/ml) $411 
MYB / c-Myb Antibody - 1:100 staining human Testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
MYB / c-Myb Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
MYB / c-Myb Antibody - Western blot analysis of Phospho-Myb (Ser532) antibody expression in mouse lung and mouse spleen tissues lysates.
MYB / c-Myb Antibody - 1:100 staining human Testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
MYB / c-Myb Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
MYB / c-Myb Antibody - Western blot analysis of Phospho-Myb (Ser532) antibody expression in mouse lung and mouse spleen tissues lysates.
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Polyclonal Rabbit anti‑Human MYB / c‑Myb Antibody (phospho‑Ser532, IHC, IF, WB) LS‑C800996

Polyclonal Rabbit anti‑Human MYB / c‑Myb Antibody (phospho‑Ser532, IHC, IF, WB) LS‑C800996

Antibody:
MYB / c-Myb Rabbit anti-Human Polyclonal (pSer532) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$369
LS-C800996-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
MYB / c-Myb Rabbit anti-Human Polyclonal (pSer532) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
C-Myb antibody LS-C800996 is an unconjugated rabbit polyclonal antibody to c-Myb (MYB) (pSer532) from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB.
Target
Human MYB / c-Myb
Synonyms
MYB | C-myb_CDS | C-myb8B_CDS | C-myb protein (140 AA) | C-myb10A_CDS | C-myb13A_CDS | Efg | Transcriptional activator Myb | C-myb | C-myb14A_CDS | Cmyb | Proto-oncogene c-Myb
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Myb around the phosphorylation site of Serine 532.
Epitope
pSer532
Specificity
Phospho-Myb (Ser532) Antibody detects endogenous levels of Myb only when phosphorylated at Serine 532.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MYB / c-Myb
P10242 NM_005375 NP_005366.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/25/2022