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Catalog Number Size Price
LS-C745320-25 25 µl (1 mg/ml) $275 
MYB / c-Myb Antibody - Western Blot of rabbit anti-C-Myb antibody. Lane 1: Cos7 cell lysates vector. Lane 2: Cos7 cell lysates transfected with c-myb, no treatment. Lane 3: Cos7 cell lysates transfected with c-Myb and heat stress-treated (incubation at 42°C for 30 minutes) to induce c-Myb modification. Load: 35 µg per lane. Primary antibody: cMyb antibody at 1:500 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 10% BLOTTO overnight at 4°C. Predicted/Observed size: Lane 3 shows detection of bands at 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrows): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively.

Polyclonal Rabbit anti‑Mouse MYB / c‑Myb Antibody (Internal, WB) LS‑C745320

Polyclonal Rabbit anti‑Mouse MYB / c‑Myb Antibody (Internal, WB) LS‑C745320

Antibody:
MYB / c-Myb Rabbit anti-Mouse Polyclonal (Internal) Antibody
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745320-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
MYB / c-Myb Rabbit anti-Mouse Polyclonal (Internal) Antibody
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
C-Myb antibody LS-C745320 is an unconjugated rabbit polyclonal antibody to mouse c-Myb (MYB) (Internal). Validated for ELISA and WB.
Target
Mouse MYB / c-Myb
Synonyms
MYB | C-myb_CDS | C-myb8B_CDS | C-myb protein (140 AA) | C-myb10A_CDS | C-myb13A_CDS | Efg | Transcriptional activator Myb | C-myb | C-myb14A_CDS | Cmyb | Proto-oncogene c-Myb
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Predicted
Human, Chimpanzee, Gorilla, Orangutan, Rat, Horse, Opossum, Pig, Platypus, Spider monkey (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of mouse c-Myb protein.
Epitope
Internal
Specificity
This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells. A BLAST analysis was used to suggest reactivity with c-Myb from mouse based on a 100% homology with the immunizing sequence. Expect reactivity with c-Myb from rat, pig, human, horse, platypus, opossum, orangutan, spider monkey, bonobo, gorilla, and chimpanzee; with a v-myb myeloblastosis viral oncogene homolog, all isoforms (also avian version) from human; and with a protooncogene c-myb, and a v-myb myeloblastosis viral oncogene homolog (avian), both from cattle, all based on a 93% homology with the immunizing sequence. Cross-reactivity with c-Myb from other sources has not been determined.
Applications
  • Western blot (1:250 - 1:500)
  • ELISA (1:10000 - 1:25000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MYB / c-Myb
P10242 NM_005375 NP_005366.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/6/2021