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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MGMT Antibody (clone MT 23.2) LS‑C2710
MGMT antibody LS-C2710 is an unconjugated mouse monoclonal antibody to human MGMT. Validated for ICC, IF, IHC and WB.
Catalog
Size
Price
LS-C2710-100
100 µl
$335

Popular MGMT Products

Formalin-fixed, paraffin-embedded human colon carcinoma stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Hamster
Applications: IHC, IHC - Paraffin, Western blot
Formalin-fixed and paraffin-embedded human placenta with MGMT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Formalin-fixed, paraffin-embedded human colon carcinoma stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Hamster
Applications: IHC, IHC - Paraffin, Western blot
MGMT antibody LS-C116198 Western blot of A549 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Hamster, Horse, Pig
Applications: Western blot
Human Small Intestine, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

MGMT antibody LS-C2710 is an unconjugated mouse monoclonal antibody to human MGMT. Validated for ICC, IF, IHC and WB.

Specifications

Target
Human MGMT
Synonyms
MGMT
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse
Clonality
IgG2b Monoclonal [MT 23.2]
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC (1:2000)
  • ICC
  • Immunofluorescence
  • Western blot (1:1000 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MGMT antibody was raised against recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli
Specificity
MGMT.
Presentation
0.1% Sodium Azide
Storage
Short term: -20°C; Long term: -70°C.
Restrictions
For research use only.
About MGMT
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. P16455 NM_002412 NP_002403.2


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Images

Immunofluorescence

Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) - Detection of MGMT (Green) in Hela cells at a 1:50 dilution.  Nuclei (Blue) are counterstained using Hoechst 33258.
Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) - Detection of MGMT (Green) in Hela cells at a 1:50 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.

Western blot

MGMT antibody (MT 23.2) - Detection of MGMT in CEM whole cell extract using MGMT Antibody LS-C2710 at 1:100 dilution.
MGMT antibody (MT 23.2) - Detection of MGMT in CEM whole cell extract using MGMT Antibody LS-C2710 at 1:100 dilution.

Western blot

MGMT Antibody (MT 23.2) - Western blot of MGMT (MT 23.2) antibody (MGMT Antibody LS-C2710) - Detection of MGMT in MCF7 lysate at 1:1000 dilution.
MGMT Antibody (MT 23.2) - Western blot of MGMT (MT 23.2) antibody (MGMT Antibody LS-C2710) - Detection of MGMT in MCF7 lysate at 1:1000 dilution.

Immunofluorescence

Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) - Detection of MGMT (Green) in Hela cells at a 1:50 dilution.  Nuclei (Blue) are counterstained using Hoechst 33258.
Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) - Detection of MGMT (Green) in Hela cells at a 1:50 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.

Western blot

MGMT antibody (MT 23.2) - Detection of MGMT in CEM whole cell extract using MGMT Antibody LS-C2710 at 1:100 dilution.
MGMT antibody (MT 23.2) - Detection of MGMT in CEM whole cell extract using MGMT Antibody LS-C2710 at 1:100 dilution.

Western blot

MGMT Antibody (MT 23.2) - Western blot of MGMT (MT 23.2) antibody (MGMT Antibody LS-C2710) - Detection of MGMT in MCF7 lysate at 1:1000 dilution.
MGMT Antibody (MT 23.2) - Western blot of MGMT (MT 23.2) antibody (MGMT Antibody LS-C2710) - Detection of MGMT in MCF7 lysate at 1:1000 dilution.

Immunofluorescence

Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) - Detection of MGMT (Green) in Hela cells at a 1:50 dilution.  Nuclei (Blue) are counterstained using Hoechst 33258.
Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) - Detection of MGMT (Green) in Hela cells at a 1:50 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.

Western blot

MGMT antibody (MT 23.2) - Detection of MGMT in CEM whole cell extract using MGMT Antibody LS-C2710 at 1:100 dilution.
MGMT antibody (MT 23.2) - Detection of MGMT in CEM whole cell extract using MGMT Antibody LS-C2710 at 1:100 dilution.

Western blot

MGMT Antibody (MT 23.2) - Western blot of MGMT (MT 23.2) antibody (MGMT Antibody LS-C2710) - Detection of MGMT in MCF7 lysate at 1:1000 dilution.
MGMT Antibody (MT 23.2) - Western blot of MGMT (MT 23.2) antibody (MGMT Antibody LS-C2710) - Detection of MGMT in MCF7 lysate at 1:1000 dilution.

Immunofluorescence

Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) - Detection of MGMT (Green) in Hela cells at a 1:50 dilution.  Nuclei (Blue) are counterstained using Hoechst 33258.
Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) - Detection of MGMT (Green) in Hela cells at a 1:50 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.

Western blot

MGMT antibody (MT 23.2) - Detection of MGMT in CEM whole cell extract using MGMT Antibody LS-C2710 at 1:100 dilution.
MGMT antibody (MT 23.2) - Detection of MGMT in CEM whole cell extract using MGMT Antibody LS-C2710 at 1:100 dilution.

Western blot

MGMT Antibody (MT 23.2) - Western blot of MGMT (MT 23.2) antibody (MGMT Antibody LS-C2710) - Detection of MGMT in MCF7 lysate at 1:1000 dilution.
MGMT Antibody (MT 23.2) - Western blot of MGMT (MT 23.2) antibody (MGMT Antibody LS-C2710) - Detection of MGMT in MCF7 lysate at 1:1000 dilution.

Requested From: United States
Date Requested: 10/19/2018

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