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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MGMT

O-6-methylguanine-DNA methyltransferase

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Gene Name: O-6-methylguanine-DNA methyltransferase
Synonyms: MGMT
Target Sequences: NM_002412 NP_002403.2 P16455

Publications (7)

1
Differential degradation rates of inactivated alkyltransferase in blood mononuclear cells and tumors of patients after treatment with O(6)-benzylguanine. Liu L, Spiro TP, Qin X, Majka S, Haaga J, Schupp J, Willson JK, Gerson SL. Clinical cancer research : an official journal of the American Association for Cancer Research. 2001 7:2318-24. (IHC; Human) [PubMed:11489807]
2
Human-yeast chimeric repair protein protects mammalian cells against alkylating agents: enhancement of MGMT protection. Roth TJ, Xu Y, Luo M, Kelley MR. Cancer gene therapy. 2003 10:603-10. (WB) [PubMed:12872142]
3
O6-methylguanine-DNA methyltransferase gene: epigenetic silencing and prognostic value in head and neck squamous cell carcinoma. Zuo C, Ai L, Ratliff P, Suen JY, Hanna E, Brent TP, Fan CY. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. 2004 13:967-75. (IHC; Human) [PubMed:15184253]
4
Mitochondrial targeting of human O6-methylguanine DNA methyltransferase protects against cell killing by chemotherapeutic alkylating agents. Cai S, Xu Y, Cooper RJ, Ferkowicz MJ, Hartwell JR, Pollok KE, Kelley MR. Cancer research. 2005 65:3319-27. (WB) [PubMed:15833865]
5
MGMT expression and promoter methylation status may depend on the site of surgical sample collection within glioblastoma: a possible pitfall in stratification of patients?. Della Puppa A, Persano L, Masi G, Rampazzo E, Sinigaglia A, Pistollato F, Denaro L, Barzon L, Pal G, Basso G, Scienza R, d'Avella D. Journal of neuro-oncology. 2012 106:33-41. [PubMed:21725802]
6
Human O(6) -methylguanine-DNA methyltransferase containing C145A does not prevent hepatocellular carcinoma in C3HeB/FeJ transgenic mice. Herzig MC, Hildreth K, Huamani J, Perez M, Goins BA, McMahan CA, Reddick RL, Walter CA. Molecular carcinogenesis. 2013 52:275-85. (WB; Mouse) [PubMed:22213062]
7
[Induction of repair enzyme O6-methylguanine-DNA methyltransferase gene expression under the influence of cytokine EMAP II in human cells in vitro]. Lylo VV, Matsevich LL, Kotsarenko EV, Babenko LA, Korneliuk AI, Sukhorada EM, Lukash LL. TSitologiia i genetika. 45:53-60. [PubMed:22329163]
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Filtered by: None
Target
MGMT (126)
Reactivity
Human (114)
Mouse (23)
Rat (28)
Bat (4)
Chimpanzee (3)
Dog (4)
Gibbon (3)
Guinea pig (4)
Hamster (7)
Horse (4)
Monkey (8)
Pig (4)
Rabbit (1)
Application
IHC (65)
IHC-P (15)
WB (118)
Flo (19)
ELISA (27)
ICC (10)
IF (28)
IP (9)
Peptide-ELISA (2)
Host
rabbit (66)
mouse (60)
Isotype
Ig (1)
IgG (32)
IgG1 (43)
IgG1,k (1)
IgG2b (10)
IgG3 (5)
Clonality
monoclonal mc (59)
polyclonal pc (67)
Clone
1527CT158.87.44 (1)
1A11A8 (1)
1A11F8 (1)
888CT22.2.4 (1)
MT 23.2 (1)
MT 3.1 (3)
MT3.1 (1)
OTI10H9 (2)
OTI17H4 (2)
OTI1B12 (2)
OTI2E2 (2)
OTI2F10 (2)
OTI4D5 (2)
OTI4E6 (2)
OTI4F9 (2)
OTI5A7 (2)
OTI6C4 (2)
OTI6G6 (2)
UMAB52 (2)
UMAB56 (2)
Format
AP Conjugated (5)
APC (2)
APC Conjugated (7)
Azide-free (3)
Biotin Conjugated (9)
Carrier-free (13)
Cy3 Conjugated (2)
Cy7 Conjugated (2)
FITC Conjugated (9)
HRP Conjugated (9)
PE Conjugated (7)
Unconjugated (76)
Epitope
aa1-30 (9)
aa1-207 (8)
aa1-209 (8)
aa1-100 (5)
aa143-192 (4)
1-80 aa, N-terminal (1)
Cys5 (1)
N-Terminus (1)
aa1-50 (1)
aa156-182 (1)
aa2-207 (1)
aa8 (1)
aa8-221 (1)
aa87-101 (1)
Publications
No (126)
Showing 1-25 of 126 results

IHC-plus™ MGMT Antibody LS-B13847
Rabbit Polyclonal (IgG) to Human MGMT
Mouse, Rat, Human
IF, IHC, IHC-P, WB
Unconjugated
50µl  $425.00
Human Small Intestine, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of U2OS cells using MGMT antibody. Blue: DAPI for nuclear staining.

MGMT Antibody (aa156-182) LS-C100520
Rabbit Polyclonal to Human MGMT
Human
Flo, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human placenta with MGMT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
MGMT Antibody western blot of Jurkat cell line lysates (35 ug/lane). The MGMT antibody detected the MGMT protein (arrow).

MGMT Antibody (clone MT 3.1) LS-C88207
Mouse Monoclonal [clone MT 3.1] (IgG1) to Human MGMT
Mouse, Rat, Hamster, Human
IHC, IHC-P, WB
Unconjugated
100µl 0.4mg/ml  $305.00; 500µl 200µg/ml  $620.00; 1ml 200µg/ml  $1,020.00
Formalin-fixed, paraffin-embedded human colon carcinoma stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.

MGMT Antibody (clone MT 3.1, Biotin) LS-C88208
Mouse Monoclonal [clone MT 3.1] (IgG1) to Human MGMT
Mouse, Rat, Hamster, Human
IHC, IHC-P, WB
Biotin Conjugated
100µl 200µg/ml  $355.00; 500µl 200µg/ml  $800.00; 1ml 200µg/ml  $1,275.00
Formalin-fixed, paraffin-embedded human colon carcinoma stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.

MGMT Antibody LS-C9010
Mouse Monoclonal (IgG1) to Human MGMT
Human
Flo, IF, IHC, IHC-P, IP, WB
Unconjugated
100µg  $650.00

MGMT Antibody (HRP) LS-C547994
Mouse Monoclonal (IgG1) to Human MGMT
Human
Flo, IF, IHC, IP, WB
HRP Conjugated
100µl  $830.00

MGMT Antibody (Biotin) LS-C527895
Mouse Monoclonal (IgG1) to Human MGMT
Human
Flo, IF, IHC, IP, WB
Biotin Conjugated
100µl  $830.00

MGMT Antibody (FITC) LS-C537856
Mouse Monoclonal (IgG1) to Human MGMT
Human
Flo, IF, IHC, IP, WB
FITC Conjugated
100µl  $830.00

MGMT Antibody (PE) LS-C656024
Mouse Monoclonal (IgG1) to Human MGMT
Human
Flo, IF, IHC, IP, WB
PE Conjugated
100µl  $830.00

MGMT Antibody (APC) LS-C518132
Mouse Monoclonal (IgG1) to Human MGMT
Human
Flo, IF, IHC, IP, WB
APC Conjugated
100µl  $830.00

MGMT Antibody (clone MT 23.2) LS-C2710
Mouse Monoclonal [clone MT 23.2] (IgG2b) to Human MGMT
Human
ICC, IF, IHC, WB
Unconjugated
100µl  $335.00
Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) - Detection of MGMT (Green) in Hela cells at a 1:50 dilution.  Nuclei (Blue) are counterstained using Hoechst 33258.
MGMT Antibody (MT 23.2) - Western blot of MGMT (MT 23.2) antibody (MGMT Antibody MGMT antibody) - Detection of MGMT in MCF7 lysate at 1:1000 dilution.

MGMT Antibody (clone 1A11F8) LS-C669307
Mouse Monoclonal [clone 1A11F8] (IgG1) to Human MGMT
Human
ELISA, Flo, IF, IHC, WB
Unconjugated
0.1mg  $365.00

MGMT Antibody (N-Terminus) LS-C413105
Rabbit Polyclonal to Human MGMT
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $275.00
Immunohistochemical analysis of MGMT staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of MGMT staining in T98G cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

MGMT Antibody LS-C801400
Rabbit Polyclonal (IgG) to Human MGMT
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Immunofluorescence Image

MGMT Antibody (aa143-192) LS-C116198
Rabbit Polyclonal to Human MGMT
Mouse, Dog, Guinea pig, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
MGMT antibody Western blot of A549 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

MGMT Antibody (aa2-207) LS-C357431
Rabbit Polyclonal to Human MGMT
Human
ICC, IHC, IHC-P, WB
Unconjugated
100µg 200µl 0.5mg/ml  $325.00
MGMT antibody IHC-paraffin: Human Placenta Tissue.
ICC analysis of MGMT using anti-MGMT antibody. MGMT was detected in immunocytochemical section of human MCF-7 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-MGMT Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

MGMT Antibody (clone UMAB52) LS-C796817
Mouse Monoclonal [clone UMAB52] (IgG1) to Human MGMT
Human, Monkey
IF, IHC, WB
Unconjugated
100µl  $395.00
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MGMT mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-MGMT mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

MGMT Antibody (clone UMAB52, Carrier-free) LS-C796818
Mouse Monoclonal [clone UMAB52] (IgG1) to Human MGMT
Human, Monkey
IF, IHC, WB
Unconjugated, Carrier-free
100µg  $425.00
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MGMT mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-MGMT mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

MGMT Antibody (clone UMAB56) LS-C799236
Mouse Monoclonal [clone UMAB56] (IgG1) to Human MGMT
Human, Monkey
IF, IHC, WB
Unconjugated
100µl  $395.00
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-MGMT mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MGMT mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

MGMT Antibody (clone UMAB56, Carrier-free) LS-C796820
Mouse Monoclonal [clone UMAB56] (IgG1) to Human MGMT
Human, Monkey
IF, IHC, WB
Unconjugated, Carrier-free
100µg  $425.00
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-MGMT mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MGMT mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

MGMT Antibody LS-C9013
Rabbit Polyclonal to Human MGMT
ELISA, IF, IHC, WB
Unconjugated
500µg  $710.00

MGMT Antibody (aa8-221) LS-C9009
Mouse Monoclonal (IgG1) to Human MGMT
Human
Flo, IHC, IHC-P, WB
Unconjugated
500µl  $680.00

MGMT Antibody LS-C9018
Mouse Monoclonal (IgG2b) to Human MGMT
Human
Flo, IHC, IHC-P, WB
Unconjugated
100µg  $635.00

IHC-plus™ MGMT Antibody LS-B15049
Rabbit Polyclonal (IgG) to Human MGMT
Human
IHC, IHC-P, WB
Unconjugated
50µl  $425.00
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of extracts of Jurkat cells at 1:1000 dilution.

MGMT Antibody (clone MT 3.1, Azide-free) LS-C88209
Mouse Monoclonal [clone MT 3.1] (IgG1) to Human MGMT
Mouse, Rat, Hamster, Human
Flo, IHC, IHC-P, WB
Unconjugated, Azide-free
100µl 1mg/ml  $680.00; 200µl 1mg/ml  $1,110.00
Formalin-fixed, paraffin-embedded human colon carcinoma stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Showing 1-25 of 126 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).