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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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MGMT

O-6-methylguanine-DNA methyltransferase

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Gene Name: O-6-methylguanine-DNA methyltransferase
Synonyms: MGMT
Target Sequences: NM_002412 NP_002403.2 P16455

Publications (7)

1
Differential degradation rates of inactivated alkyltransferase in blood mononuclear cells and tumors of patients after treatment with O(6)-benzylguanine. Liu L, Spiro TP, Qin X, Majka S, Haaga J, Schupp J, Willson JK, Gerson SL. Clinical cancer research : an official journal of the American Association for Cancer Research. 2001 7:2318-24. (IHC; Human) [PubMed:11489807]
2
Human-yeast chimeric repair protein protects mammalian cells against alkylating agents: enhancement of MGMT protection. Roth TJ, Xu Y, Luo M, Kelley MR. Cancer gene therapy. 2003 10:603-10. (WB) [PubMed:12872142]
3
O6-methylguanine-DNA methyltransferase gene: epigenetic silencing and prognostic value in head and neck squamous cell carcinoma. Zuo C, Ai L, Ratliff P, Suen JY, Hanna E, Brent TP, Fan CY. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. 2004 13:967-75. (IHC; Human) [PubMed:15184253]
4
Mitochondrial targeting of human O6-methylguanine DNA methyltransferase protects against cell killing by chemotherapeutic alkylating agents. Cai S, Xu Y, Cooper RJ, Ferkowicz MJ, Hartwell JR, Pollok KE, Kelley MR. Cancer research. 2005 65:3319-27. (WB) [PubMed:15833865]
5
MGMT expression and promoter methylation status may depend on the site of surgical sample collection within glioblastoma: a possible pitfall in stratification of patients?. Della Puppa A, Persano L, Masi G, Rampazzo E, Sinigaglia A, Pistollato F, Denaro L, Barzon L, Pal G, Basso G, Scienza R, d'Avella D. Journal of neuro-oncology. 2012 106:33-41. [PubMed:21725802]
6
Human O(6) -methylguanine-DNA methyltransferase containing C145A does not prevent hepatocellular carcinoma in C3HeB/FeJ transgenic mice. Herzig MC, Hildreth K, Huamani J, Perez M, Goins BA, McMahan CA, Reddick RL, Walter CA. Molecular carcinogenesis. 2013 52:275-85. (WB; Mouse) [PubMed:22213062]
7
[Induction of repair enzyme O6-methylguanine-DNA methyltransferase gene expression under the influence of cytokine EMAP II in human cells in vitro]. Lylo VV, Matsevich LL, Kotsarenko EV, Babenko LA, Korneliuk AI, Sukhorada EM, Lukash LL. TSitologiia i genetika. 45:53-60. [PubMed:22329163]
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Viewing 1-25 of 125 items
MGMT Antibody - Human Small Intestine, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE)
Select
Rabbit Polyclonal (IgG) to Human MGMT
Mouse, Rat, Human
IF, IHC, IHC-P, WB
Unconjugated
50µl   $425.00
MGMT Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Select
Rabbit Polyclonal (IgG) to Human MGMT
Human
IHC, IHC-P, WB
Unconjugated
50µl   $425.00
MGMT Antibody - Formalin-fixed and paraffin-embedded human placenta with MGMT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Select
Rabbit Polyclonal to Human MGMT
Human
Flo, IHC, IHC-P, WB
Unconjugated
400µl   $325.00
MGMT Antibody - Formalin-fixed, paraffin-embedded human colon carcinoma stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Select
Mouse Monoclonal [clone MT 3.1] (IgG1) to Human MGMT
Mouse, Rat, Hamster, Human
IHC, IHC-P, WB
Unconjugated
100µl   0.4mg/ml   $305.00; 500µl   200µg/ml   $620.00; 1ml   200µg/ml   $1,020.00
MGMT Antibody - Formalin-fixed, paraffin-embedded human colon carcinoma stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Select
Mouse Monoclonal [clone MT 3.1] (IgG1) to Human MGMT
Mouse, Rat, Hamster, Human
IHC, IHC-P, WB
Biotin Conjugated
100µl   200µg/ml   $355.00; 500µl   200µg/ml   $800.00; 1ml   200µg/ml   $1,275.00
Select
Mouse Monoclonal (IgG1) to Human MGMT
Human
Flo, IF, IHC, IHC-P, IP, WB
Unconjugated
100µg   $650.00
Select
Mouse Monoclonal (IgG1) to Human MGMT
Human
Flo, IF, IHC, IP, WB
HRP Conjugated
100µl   $830.00
Select
Mouse Monoclonal (IgG1) to Human MGMT
Human
Flo, IF, IHC, IP, WB
Biotin Conjugated
100µl   $830.00
Select
Mouse Monoclonal (IgG1) to Human MGMT
Human
Flo, IF, IHC, IP, WB
FITC Conjugated
100µl   $830.00
Select
Mouse Monoclonal (IgG1) to Human MGMT
Human
Flo, IF, IHC, IP, WB
PE Conjugated
100µl   $830.00
Select
Mouse Monoclonal (IgG1) to Human MGMT
Human
Flo, IF, IHC, IP, WB
APC Conjugated
100µl   $830.00
MGMT Antibody - Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) - Detection of MGMT (Green) in Hela cells at a 1:50 dilution.  Nuclei (Blue) are counterstained using Hoechst 33258.
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Mouse Monoclonal [clone MT 23.2] (IgG2b) to Human MGMT
Human
ICC, IF, IHC, WB
Unconjugated
100µl   $335.00
Select
Mouse Monoclonal [clone 1A11F8] (IgG1) to Human MGMT
Human
ELISA, Flo, IF, IHC, WB
Unconjugated
100µl   $365.00
MGMT Antibody - Immunohistochemical analysis of MGMT staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Rabbit Polyclonal to Human MGMT
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $275.00
MGMT Antibody - 1:100 staining mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
Rabbit Polyclonal (IgG) to Human MGMT
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
MGMT Antibody - MGMT antibody Western blot of A549 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Human MGMT
Mouse, Dog, Guinea pig, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
WB
Unconjugated
100µl   0.5mg/ml   $365.00
MGMT Antibody - MGMT antibody IHC-paraffin: Human Placenta Tissue.
Select
Rabbit Polyclonal to Human MGMT
Human
ICC, IHC, IHC-P, WB
Unconjugated
100µg   0.5mg/ml   $355.00
MGMT Antibody - Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-MGMT mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Select
Mouse Monoclonal [clone UMAB56] (IgG1) to Human MGMT
Human, Monkey
IF, IHC, WB
Unconjugated
100µl   1mg/ml   $395.00
MGMT Antibody - Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-MGMT mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Select
Mouse Monoclonal [clone UMAB56] (IgG1) to Human MGMT
Human, Monkey
IF, IHC, WB
Unconjugated, Carrier-free
100µg   $425.00
MGMT Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MGMT mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Select
Mouse Monoclonal [clone UMAB52] (IgG1) to Human MGMT
Human, Monkey
IF, IHC, WB
Unconjugated
100µl   1mg/ml   $395.00
MGMT Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MGMT mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Select
Mouse Monoclonal [clone UMAB52] (IgG1) to Human MGMT
Human, Monkey
IF, IHC, WB
Unconjugated, Carrier-free
100µg   $425.00
Select
Rabbit Polyclonal to Human MGMT
ELISA, IF, IHC, WB
Unconjugated
500µg   $710.00
Select
Mouse Monoclonal (IgG1) to Human MGMT
Human
Flo, IHC, IHC-P, WB
Unconjugated
500µl   $680.00
Select
Mouse Monoclonal (IgG2b) to Human MGMT
Human
Flo, IHC, IHC-P, WB
Unconjugated
100µg   $635.00
MGMT Antibody - Formalin-fixed, paraffin-embedded human colon carcinoma stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Select
Mouse Monoclonal [clone MT 3.1] (IgG1) to Human MGMT
Mouse, Rat, Hamster, Human
Flo, IHC, IHC-P, WB
Unconjugated, Azide-free
100µl   1mg/ml   $680.00; 200µl   1mg/ml   $1,110.00

Viewing 1-25 of 125 items


If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).