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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C15051-100 100 µl $635 
Antibody

Polyclonal Rabbit anti‑Human MAPK3 / ERK1 Antibody (phospho‑Thr202/Tyr204, IHC, WB) LS‑C15051

Polyclonal Rabbit anti‑Human MAPK3 / ERK1 Antibody (phospho‑Thr202/Tyr204, IHC, WB) LS‑C15051

Antibody:
Rabbit Polyclonal to Human MAPK3 / ERK1
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$635 
LS-C15051-100
100 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human MAPK3 / ERK1
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ERK1 antibody LS-C15051 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) (pThr202/Tyr204) from human. It is reactive with human and rat. Validated for IHC and WB.
Target
Human MAPK3 / ERK1
Synonyms
MAPK3 | ERK-1 | ERT2 | HUMKER1A | HS44KDAP | MAP kinase 1 | MAP kinase isoform p44 | MAPK 1 | MAPK 3 | p44 | p44MAPK | p44-MAPK | p44ERK1 | MAP kinase 3 | p44-ERK1 | ERK1 | PRKM3
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic phosphopeptide corresponding to the regions surrounding the phosphorylated Thr202 and Tyr204 residues of ERK1+2 (p44/42 MAP kinase).
Epitope
pThr202/Tyr204
Specificity
Specific for ERK 1+2 when phosphorylated at Thr202 and Tyr204. On Western blots, it identifies two bands at ~42 and 44kD. Reactivity has been confirmed with UV-treated human Jurkat cell lysates. Immunolabeling in UV-treated Jurkat cells is blocked by the phosphopeptide, but not by the dephosphopeptide.
Usage
Suitable for use in Western Blot and Immunohistochemistry. Western Blot (rat): 1:1000. Immunohistochemistry, human (formalin fixed, paraffin embedded): 1:400. Staining of formalin-fixed, paraffin-embedded tissues, heat induced epitope retrieval with citrate buffer, pH 6.0, is required prior to staining.
Presentation
10 mM HEPES, pH 7.5, 0.1 mg/ml BSA, 50% Glycerol
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPK3 / ERK1
P27361 X60188 CAA42744.1

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Requested From: United States
Date Requested: 7/5/2020