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Catalog Number Size Price
LS-A3520-50 50 µg (1 mg/ml) $460 
MAPK3 / ERK1 Antibody - Anti-MAPK3 / ERK1 antibody IHC of human neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

PathPlus™ Polyclonal Rabbit anti‑Human MAPK3 / ERK1 Antibody (Internal, IHC) LS‑A3520

PathPlus™ Polyclonal Rabbit anti‑Human MAPK3 / ERK1 Antibody (Internal, IHC) LS‑A3520

Antibody:
MAPK3 / ERK1 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$460
LS-A3520-50

Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MAPK3 / ERK1 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
MAPK3 is a serine/threonine kinase that is an essential member of the MAP kinase signal transduction pathway (MAPK/ERK cascade). MAPK3 is required for proper targeting of presynaptic axons to postsynaptic targets, and MAPK3 mutations or dysfunction may induce the formation of abnormal neuronal architecture and contribute to the pathogenesis of autism spectrum disorder and other neuropsychiatric disorders. In immunohistochemistry, MAPK3 has cytoplasmic positivity in cells throughout the central nervous system and is found in most other tissues in the body. References: Mol Cells. 2017 Feb 28; 40(2): 151–161, PMID: 28196412; Int J Dev Neurosci. 2014 Dec;39:2-8, PMID: 24631207;
Target
Human MAPK3 / ERK1
Synonyms
MAPK3 | ERK-1 | ERT2 | HUMKER1A | HS44KDAP | MAP kinase 1 | MAP kinase isoform p44 | MAPK 1 | MAPK 3 | p44 | p44MAPK | p44-MAPK | p44ERK1 | MAP kinase 3 | p44-ERK1 | ERK1 | PRKM3
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
Predicted
Bat, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 18 amino acid peptide from internal region of human MAPK3 / ERK1. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Hamster, Panda, Horse, Pig (100%); Bat, Elephant, Rabbit (94%).
Epitope
Internal
Specificity
Human MAPK3 / ERK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MAPK1 (61%).
Applications
  • IHC
  • IHC - Paraffin (7 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 7 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPK3 / ERK1
P27361 X60188 CAA42744.1

Validation

MAPK3 / ERK1 Antibody - Anti-MAPK3 / ERK1 antibody IHC of human neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-MAPK3 / ERK1 antibody IHC of human neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-MAPK3 / ERK1 antibody IHC of human neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

PathPlus™ MAPK3 / ERK1 Antibody (Internal) for IHC LS-A3520 has an LSBio Rating of
Laboratory Validation Score (5)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/13/2022