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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C15048-10 10 blot $635 
Antibody

Polyclonal Rabbit anti‑Human MAPK3 / ERK1 Antibody (phospho‑Thr185/Tyr187, WB) LS‑C15048

Polyclonal Rabbit anti‑Human MAPK3 / ERK1 Antibody (phospho‑Thr185/Tyr187, WB) LS‑C15048

Antibody:
Rabbit Polyclonal to Human MAPK3 / ERK1
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$635 
LS-C15048-10
10 blot
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human MAPK3 / ERK1
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ERK1 antibody LS-C15048 is an unconjugated rabbit polyclonal antibody to human ERK1 (MAPK3) (pThr185/Tyr187). Validated for WB.
Target
Human MAPK3 / ERK1
Synonyms
MAPK3 | ERK-1 | ERT2 | HUMKER1A | HS44KDAP | MAP kinase 1 | MAP kinase isoform p44 | MAPK 1 | MAPK 3 | p44 | p44MAPK | p44-MAPK | p44ERK1 | MAP kinase 3 | p44-ERK1 | ERK1 | PRKM3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
Immunogen
Synthetic peptide from human ERK1/ERK2.
Epitope
pThr185/Tyr187
Specificity
Synthetic phosphopeptide derived from the region of human ERK1&2 that contains threonine 202/185 and tyrosine 204/187. This region is
Usage
The antibody Peptide Competition. Extracts prepared from PC12 cells left unstimulated or stimulated with sorbitol. were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to. PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4?C, then. were incubated with ERK1&2 [pTpY185/187] antibody for two hours at room. temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide., the non-phosphopeptide corresponding to the immunogen, a generic. phosphothreonine-containing peptide, a generic phosphotyrosine-containing. peptide, or, the phosphopeptide immunogen. After washing, membranes were. incubated with goat F (ab')2 anti-rabbit IgG HRP conjugate (Cat. # ALI4404) and. bands were detected using the Pierce SuperSignal method. The data show that only the peptide corresponding to ERK1&2 [pTpY185/187] blocks. the antibody signal, thereby demonstrating the specificity of the antibody. The data. also show the induction of phosphorylation by the addition of sorbitol. y has been used for Western blot and immunostaining applications. Positive control: PC12 cells +/- Sorbitol.
Presentation
1 mg/ml BSA (IgG, Protease free), PBS (without Mg2+, Ca2+), pH 7.2, 0.1% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPK3 / ERK1
P27361 X60188 CAA42744.1

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Requested From: United States
Date Requested: 7/5/2020