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Catalog Number Size Price
LS-C800277-100 100 µl (1 mg/ml) $379 
LS-C800277-200 200 µl (1 mg/ml) $421 
MAPK3 / ERK1 Antibody - 1:50 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
MAPK3 / ERK1 Antibody - Staining LOVO cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
MAPK3 / ERK1 Antibody - Western blot analysis on COLO205 cell lysates using ERK1/2 antibody. The lane on the left is treated with the antigen-specific peptide.
MAPK3 / ERK1 Antibody - Western blot analysis of extracts of various cellslines using erk1/2 antibody.
MAPK3 / ERK1 Antibody - Western blot analysis on HeLa cell lysates using ERK1/2 antibody
MAPK3 / ERK1 Antibody - 1:50 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
MAPK3 / ERK1 Antibody - Staining LOVO cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
MAPK3 / ERK1 Antibody - Western blot analysis on COLO205 cell lysates using ERK1/2 antibody. The lane on the left is treated with the antigen-specific peptide.
MAPK3 / ERK1 Antibody - Western blot analysis of extracts of various cellslines using erk1/2 antibody.
MAPK3 / ERK1 Antibody - Western blot analysis on HeLa cell lysates using ERK1/2 antibody
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Polyclonal Rabbit anti‑Human MAPK3 / ERK1 Antibody (IHC, IF, WB) LS‑C800277

Polyclonal Rabbit anti‑Human MAPK3 / ERK1 Antibody (IHC, IF, WB) LS‑C800277

Antibody:
MAPK3 / ERK1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep, Fish, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800277-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MAPK3 / ERK1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep, Fish, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
ERK1 antibody LS-C800277 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human MAPK3 / ERK1
Synonyms
MAPK3 | ERK-1 | ERT2 | HUMKER1A | HS44KDAP | MAP kinase 1 | MAP kinase isoform p44 | MAPK 1 | MAPK 3 | p44 | p44MAPK | p44-MAPK | p44ERK1 | MAP kinase 3 | p44-ERK1 | ERK1 | PRKM3
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep, Fish, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human ERK1/2.
Specificity
ERK1/2 antibody detects endogenous levels of total ERK1/2.
Applications
  • IHC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:1000 - 1:5000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPK3 / ERK1
P27361 X60188 CAA42744.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024