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Catalog Number Size Price
LS-C165711-200 200 µl (0.5 mg/ml) $393 
MAP1LC3B / LC3B Antibody - Immunofluorescence of U251 cells, using LC3 Antibody (APG8B). U251 cells(right) were treated with Chloroquine (50 mu M,16h). Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
MAP1LC3B / LC3B Antibody - Immunofluorescence of U251 cells, using LC3 Antibody (APG8B). U251 cells(right) were treated with Chloroquine (50 mu M,16h). Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
MAP1LC3B / LC3B Antibody - Immunofluorescence of U251 cells, using LC3 Antibody (APG8B). U251 cells(right) were treated with Chloroquine (50 mu M,16h). Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
MAP1LC3B / LC3B Antibody - Western blot of anti-LC3 (APG8b) in untreated or treated HeLa cell lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).
MAP1LC3B / LC3B Antibody - Western blot of anti-LC3 (APG8b) antibody in rat brain lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in membrane fraction (P) but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).
MAP1LC3B / LC3B Antibody - Western blot of anti-LC3 (APG8b) antibody in 293 cell line lysates transiently transfected with the LC3 (APG8b) gene (2 ug/lane). Human LC3 (APG8b) (arrow) was detected using the purified antibody.
MAP1LC3B / LC3B Antibody - Western blot of lysate from mouse NIH/3T3 cell line, using LC3 Antibody (APG8B). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
MAP1LC3B / LC3B Antibody - Western blot of lysates from HepG2 cell line, untreated or treated with chloroquine, using LC3 Antibody (upper) or Beta-actin (lower).
MAP1LC3B / LC3B Antibody - Western blot of lysates from NIH/3T3 cell line, untreated or treated with chloroquine, using LC3 Antibody (APG8B) (upper) or Beta-actin (lower).
MAP1LC3B / LC3B Antibody - Western blot of lysates from HepG2, mouse NIH/3T3 cell line, untreated or treated with chloroquine, 50uM, using LC3 Antibody (APG8B) (upper) or Beta-actin (lower).
MAP1LC3B / LC3B Antibody - Immunofluorescence of U251 cells, using LC3 Antibody (APG8B). U251 cells(right) were treated with Chloroquine (50 mu M,16h). Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
MAP1LC3B / LC3B Antibody - Immunofluorescence of U251 cells, using LC3 Antibody (APG8B). U251 cells(right) were treated with Chloroquine (50 mu M,16h). Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
MAP1LC3B / LC3B Antibody - Immunofluorescence of U251 cells, using LC3 Antibody (APG8B). U251 cells(right) were treated with Chloroquine (50 mu M,16h). Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
MAP1LC3B / LC3B Antibody - Western blot of anti-LC3 (APG8b) in untreated or treated HeLa cell lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).
MAP1LC3B / LC3B Antibody - Western blot of anti-LC3 (APG8b) antibody in rat brain lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in membrane fraction (P) but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).
MAP1LC3B / LC3B Antibody - Western blot of anti-LC3 (APG8b) antibody in 293 cell line lysates transiently transfected with the LC3 (APG8b) gene (2 ug/lane). Human LC3 (APG8b) (arrow) was detected using the purified antibody.
MAP1LC3B / LC3B Antibody - Western blot of lysate from mouse NIH/3T3 cell line, using LC3 Antibody (APG8B). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
MAP1LC3B / LC3B Antibody - Western blot of lysates from HepG2 cell line, untreated or treated with chloroquine, using LC3 Antibody (upper) or Beta-actin (lower).
MAP1LC3B / LC3B Antibody - Western blot of lysates from NIH/3T3 cell line, untreated or treated with chloroquine, using LC3 Antibody (APG8B) (upper) or Beta-actin (lower).
MAP1LC3B / LC3B Antibody - Western blot of lysates from HepG2, mouse NIH/3T3 cell line, untreated or treated with chloroquine, 50uM, using LC3 Antibody (APG8B) (upper) or Beta-actin (lower).
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Polyclonal Rabbit anti‑Human MAP1LC3B / LC3B Antibody (aa1‑30, IHC, IF, WB) LS‑C165711

Polyclonal Rabbit anti‑Human MAP1LC3B / LC3B Antibody (aa1‑30, IHC, IF, WB) LS‑C165711

Antibody:
MAP1LC3B / LC3B Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C165711-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MAP1LC3B / LC3B Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
LC3B antibody LS-C165711 is an unconjugated rabbit polyclonal antibody to LC3B (MAP1LC3B) (aa1-30) from human. It is reactive with human and rat. Validated for IF, IHC and WB. Cited in 6 publications.
Target
Human MAP1LC3B / LC3B
Synonyms
MAP1LC3B | ATG8F | LC3B | MAP1A/1BLC3 | MAP1A/MAP1B LC3 B | MAP1A/MAP1B light chain 3 B | MAP1ALC3 | MAP1LC3B-a
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Predicted
Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa1-30
Specificity
This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAP1LC3B / LC3B
Q9GZQ8 NM_022818 NP_073729.1

LSBio Ratings

MAP1LC3B / LC3B Antibody (aa1-30) for IHC, IF/Immunofluorescence, WB/Western LS-C165711 has an LSBio Rating of
Publications (4.5)

Publications (6)

Active ras triggers death in glioblastoma cells through hyperstimulation of macropinocytosis. Overmeyer JH, Kaul A, Johnson EE, Maltese WA. Molecular cancer research : MCR. 2008 6:965-77.

Lysosomal degradation of endocytosed proteins depends on the chloride transport protein ClC-7. Wartosch L, Fuhrmann JC, Schweizer M, Stauber T, Jentsch TJ. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2009 23:4056-68.

Rab5 and class III phosphoinositide 3-kinase Vps34 are involved in hepatitis C virus NS4B-induced autophagy. Su WC, Chao TC, Huang YL, Weng SC, Jeng KS, Lai MM. Journal of virology. 2011 85:10561-71.

Caspase-6 activity in a BACHD mouse modulates steady-state levels of mutant huntingtin protein but is not necessary for production of a 586 amino acid proteolytic fragment. Gafni J, Papanikolaou T, Degiacomo F, Holcomb J, Chen S, Menalled L, Kudwa A, Fitzpatrick J, Miller S, Ramboz S, Tuunanen PI, Lehtimki KK, Yang XW, Park L, Kwak S, Howland D, Park H, Ellerby LM. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2012 32:7454-65.

Chronic autophagy is a cellular adaptation to tumor acidic pH microenvironments. Wojtkowiak JW, Rothberg JM, Kumar V, Schramm KJ, Haller E, Proemsey JB, Lloyd MC, Sloane BF, Gillies RJ. Cancer research. 2012 72:3938-47.

Induction of autophagy by Imatinib sequesters Bcr-Abl in autophagosomes and down-regulates Bcr-Abl protein. Gafni J, Papanikolaou T, Degiacomo F, Holcomb J, Chen S, Menalled L, Kudwa A, Fitzpatrick J, Miller S, Ramboz S, Tuunanen PI, Lehtimki KK, Yang XW, Park L, Kwak S, Howland D, Park H, Ellerby LM. American journal of hematology. 2013

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Requested From: United States
Date Requested: 5/21/2024