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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B9581-200 200 µl $425 
MAP1LC3B / LC3B Antibody - Anti-MAP1LC3B / LC3B antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
MAP1LC3B / LC3B Antibody - Fluorescent image of U251 cells stained with APG8a/b (MAP1LC3A/B) antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated APG8a/b (MAP1LC3A/B) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). APG8a/b (MAP1LC3A/B) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
MAP1LC3B / LC3B Antibody - APG8a/b (MAP1LC3A/B) western blot of mouse lung tissue lysates (35 ug/lane). The MAP1LC3A antibody detected the MAP1LC3A protein (arrow).
MAP1LC3B / LC3B Antibody - Anti-MAP1LC3B / LC3B antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
MAP1LC3B / LC3B Antibody - Fluorescent image of U251 cells stained with APG8a/b (MAP1LC3A/B) antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated APG8a/b (MAP1LC3A/B) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). APG8a/b (MAP1LC3A/B) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
MAP1LC3B / LC3B Antibody - APG8a/b (MAP1LC3A/B) western blot of mouse lung tissue lysates (35 ug/lane). The MAP1LC3A antibody detected the MAP1LC3A protein (arrow).
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IHCPlus™ Polyclonal Rabbit anti‑Human MAP1LC3B / LC3B Antibody (aa100‑125, IHC, IF, WB) LS‑B9581

IHCPlus™ Polyclonal Rabbit anti‑Human MAP1LC3B / LC3B Antibody (aa100‑125, IHC, IF, WB) LS‑B9581

Note: This antibody replaces LS-C156409
Antibody:
Rabbit Polyclonal to Human MAP1LC3B / LC3B
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B9581-200
200 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human MAP1LC3B / LC3B
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
LC3B antibody LS-B9581 is an unconjugated rabbit polyclonal antibody to LC3B (MAP1LC3B) (aa100-125) from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human MAP1LC3B / LC3B
Synonyms
MAP1LC3B | ATG8F | LC3B | MAP1A/1BLC3 | MAP1A/MAP1B LC3 B | MAP1A/MAP1B light chain 3 B | MAP1ALC3 | MAP1LC3B-a
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E372 - Lyophilized - 500 µg - $360.00
Immunizing peptide used to generate LS-B9581. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa100-125
Specificity
This Cleaved-APG8a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-125 amino acids from human Cleaved-APG8a/b.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAP1LC3B / LC3B
Q9GZQ8 NM_022818 NP_073729.1

Validation

MAP1LC3B / LC3B Antibody - Anti-MAP1LC3B / LC3B antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-MAP1LC3B / LC3B antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-MAP1LC3B / LC3B antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

IHC-plus™ MAP1LC3B / LC3B Antibody (aa100-125) for IHC, IF/Immunofluorescence, WB/Western LS-B9581 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 7/10/2020