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MAP1LC3B / LC3B Antibody (aa25‑121, Biotin) LS‑C83721

Catalog Number Size Price
LS-C83721-100 100 µl (1 mg/ml) $365 
MAP1LC3A antibody (5 ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cortex, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of autophagic LC3 in mouse ES cell lysate using LC3 (human) Antibody. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of LC3-1 to LC3-11) demonstrates the specificity of LC3 (human) Antibody, as there is no detection of LC3-11. Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAP1LC3A antibody (5 ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cortex, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of autophagic LC3 in mouse ES cell lysate using LC3 (human) Antibody. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of LC3-1 to LC3-11) demonstrates the specificity of LC3 (human) Antibody, as there is no detection of LC3-11. Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MAP1LC3B / LC3B Antibody (aa25‑121, Biotin) LS‑C83721

Antibody:
Rabbit Polyclonal to Human MAP1LC3B / LC3B
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse, Rat, Dog, Fish, Zebrafish
Format:
Biotin, Unmodified
Price
Catalog Number
$365 
LS-C83721-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human MAP1LC3B / LC3B
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse, Rat, Dog, Fish, Zebrafish
Format:
Biotin, Unmodified

Specifications

Description
LC3B antibody LS-C83721 is a biotin-conjugated rabbit polyclonal antibody to LC3B (MAP1LC3B ) from human, mouse, rat and other species. Validated for IHC, IP and WB.
Target
Human MAP1LC3B / LC3B
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Fish, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (2 µg/ml)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121)
Epitope
aa25-121
Specificity
This detects both LC3A and LC3B.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. This antibody is useful for Western blot and immunohistochemistry. By Western blot bands are seen at ~19 kD, representing LC3-I, and ~17 kD, representing LC3-II. There are also a few non-specific bands above ~32 kD in mouse protein.
Presentation
PBS, 0.05% Sodium Azide
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAP1LC3B / LC3B
Q9GZQ8 NM_022818 NP_073729.1

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Featured Products

Anti-MAP1LC3B / LC3B antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Pig, Zebrafish, Primate
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Flow Cytometry
Anti-MAP1LC3B / LC3B antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
MAP1LC3A antibody (5 ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cortex, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Rat, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Anti-MAP1LC3B / LC3B antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Product
Species: Human
Applications: ELISA
Reactivity: Mouse
Range: 78-5000 pg/ml

Requested From: United States
Date Requested: 9/21/2019