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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL2RA / CD25 Antibody (RPE) LS‑C45049
CD25 antibody LS-C45049 is an RPE-conjugated mouse monoclonal antibody to rat CD25 (IL2RA). Validated for Flow.
Catalog
Size
Price
LS-C45049-100
100 tst
$415
Description
CD25 antibody LS-C45049 is an RPE-conjugated mouse monoclonal antibody to rat CD25 (IL2RA). Validated for Flow.
Target
Rat IL2RA / CD25
Host
Mouse
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
R. Phycoerythrin
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Flow Cytometry (1:1)
Immunogen
IL2RA / CD25 antibody was raised against con A stimulated rat T cells.
Specificity
Recognizes the alpha chain of rat CD25, otherwise know as IL-2 receptor alpha, a 55kD type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts. XXX recommend the use of MCA273EL for this purpose.
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized PBS, pH 7.4, 0.09% Sodium Azide, 1% BSA
Reconstitution
100 tst Distilled Water.
Restrictions
For research use only.
About IL2RA / CD25
P01589 NM_000417 NP_000408.1

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Requested From: United States
Date Requested: 12/19/2018
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