(tested or 100% immunogen sequence identity)
Protein G purified
IL2RA / CD25 antibody was raised against con A stimulated rat T cells.
Recognizes the alpha chain of rat CD25, otherwise know as IL-2 receptor alpha, a 55kD type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts. XXX recommend the use of MCA273EL for this purpose.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA
Reconstitute with distilled water.
Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
For research use only.
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