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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL2RA / CD25 Antibody (aa34‑139, clone 1B5D12) LS‑C191874
CD25 antibody LS-C191874 is an unconjugated mouse monoclonal antibody to CD25 (IL2RA) from human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB.
Catalog
Size
Price
LS-C191874-100
100 µg (1 mg/ml)
$365
Description
CD25 antibody LS-C191874 is an unconjugated mouse monoclonal antibody to CD25 (IL2RA) from human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB.
Target
Human IL2RA / CD25
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [1B5D12]
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200 - 1:1000)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
IL2RA / CD25 antibody was raised against purified recombinant fragment of human IL2RA (AA: 34-139) expressed in E. Coli.
Epitope
aa34-139
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: -20°C.
Restrictions
For research use only.
About IL2RA / CD25
P01589 NM_000417 NP_000408.1

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Images

Immunohistochemistry

IHC of paraffin-embedded rectum cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded rectum cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded bladder cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded bladder cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence of HeLa cells using IL2RA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of HeLa cells using IL2RA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot using IL2RA mouse monoclonal antibody against HeLa (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.
Western blot using IL2RA mouse monoclonal antibody against HeLa (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.

Western blot

Western blot using IL2RA monoclonal antibody against human IL2RA recombinant protein. (Expected MW is 37.5 kDa)
Western blot using IL2RA monoclonal antibody against human IL2RA recombinant protein. (Expected MW is 37.5 kDa)

Immunohistochemistry

IHC of paraffin-embedded rectum cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded rectum cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded bladder cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded bladder cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence of HeLa cells using IL2RA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of HeLa cells using IL2RA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot using IL2RA mouse monoclonal antibody against HeLa (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.
Western blot using IL2RA mouse monoclonal antibody against HeLa (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.

Western blot

Western blot using IL2RA monoclonal antibody against human IL2RA recombinant protein. (Expected MW is 37.5 kDa)
Western blot using IL2RA monoclonal antibody against human IL2RA recombinant protein. (Expected MW is 37.5 kDa)

Immunohistochemistry

IHC of paraffin-embedded rectum cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded rectum cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded bladder cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded bladder cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence of HeLa cells using IL2RA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of HeLa cells using IL2RA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot using IL2RA mouse monoclonal antibody against HeLa (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.
Western blot using IL2RA mouse monoclonal antibody against HeLa (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.

Western blot

Western blot using IL2RA monoclonal antibody against human IL2RA recombinant protein. (Expected MW is 37.5 kDa)
Western blot using IL2RA monoclonal antibody against human IL2RA recombinant protein. (Expected MW is 37.5 kDa)

Immunohistochemistry

IHC of paraffin-embedded rectum cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded rectum cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded bladder cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded bladder cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence of HeLa cells using IL2RA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of HeLa cells using IL2RA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot using IL2RA mouse monoclonal antibody against HeLa (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.
Western blot using IL2RA mouse monoclonal antibody against HeLa (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.

Western blot

Western blot using IL2RA monoclonal antibody against human IL2RA recombinant protein. (Expected MW is 37.5 kDa)
Western blot using IL2RA monoclonal antibody against human IL2RA recombinant protein. (Expected MW is 37.5 kDa)

Requested From: United States
Date Requested: 12/18/2018
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