Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-B7396-500 500 µg $460 
IL2RA / CD25 Antibody - Anti-CD25 antibody IHC of human plasma cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC‑plus™ Monoclonal Mouse anti‑Human IL2RA / CD25 Antibody (IHC, WB) LS‑B7396

IHC‑plus™ Monoclonal Mouse anti‑Human IL2RA / CD25 Antibody (IHC, WB) LS‑B7396

Note: This antibody replaces LS-C104460
Antibody:
IL2RA / CD25 Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B7396-500
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IL2RA / CD25 Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD25 antibody LS-B7396 is an unconjugated mouse monoclonal antibody to human CD25 (IL2RA). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human IL2RA / CD25
Synonyms
IL2RA | CD25 antigen | CD25 | IDDM10 | IL-2 receptor subunit alpha | IL2R | Interleukin 2 receptor, alpha | IL-2-RA | IL2-RA | TCGFR | IL-2R subunit alpha | p55 | TAC antigen
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Insect cell derived recombinant Human sIL-2Ralpha.
Specificity
Human IL-2Ralpha
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen (5 µg/ml)
  • Western blot (0.2 - 0.4 µg/ml)
  • ELISA (4 - 8 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B7396 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 4-8 ug/ml of this antibody will detect at least 100 pg/ml of recombinant Human sIL-2Ralpha when used in conjunction with compatible secondary reagents. Western Blot: To detect Human sIL-2Ralpha by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human sIL-2Ralpha is 2-4 ng/lane, under non-reducing conditions.
Presentation
Lyophilized from Sterile Solution PBS
Reconstitution
Centrifuge vial prior to opening. Reconstitiute in sterile water to 1.0 mg/ml.
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL2RA / CD25
P01589 NM_000417 NP_000408.1

Validation

IL2RA / CD25 Antibody - Anti-CD25 antibody IHC of human plasma cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CD25 antibody IHC of human plasma cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CD25 antibody IHC of human plasma cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ IL2RA / CD25 Antibody for IHC, WB/Western, ELISA LS-B7396 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/13/2024