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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL2RA / CD25 Antibody (clone OX‑39, APC) LS‑C107247
CD25 antibody LS-C107247 is an APC-conjugated mouse monoclonal antibody to rat CD25 (IL2RA). Validated for Flow and IHC.
Catalog
Size
Price
LS-C107247-100
100 µg (0.2 mg/ml)
$345
Description
CD25 antibody LS-C107247 is an APC-conjugated mouse monoclonal antibody to rat CD25 (IL2RA). Validated for Flow and IHC.
Target
Rat IL2RA / CD25
Host
Mouse
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [OX-39]
Conjugations
Allophycocyanin
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
IL2RA / CD25 antibody was raised against rat IL2RA
Usage
This OX39 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.125 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.
Storage
Store at 4°C. Do not freeze. Protect from light.
Restrictions
For research use only.
About IL2RA / CD25
P01589 NM_000417 NP_000408.1

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Images

Flow Cytometry

Staining of rat splenocytes with FITC anti-rat CD4 and 0.06 ug of APC Mouse IgG1, K Isotype Control (left) or 0.06 ug of APC anti-rat CD25 (right). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of rat splenocytes with FITC anti-rat CD4 and 0.06 ug of APC Mouse IgG1, K Isotype Control (left) or 0.06 ug of APC anti-rat CD25 (right). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of rat splenocytes with FITC anti-rat CD4 and 0.06 ug of APC Mouse IgG1, K Isotype Control (left) or 0.06 ug of APC anti-rat CD25 (right). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of rat splenocytes with FITC anti-rat CD4 and 0.06 ug of APC Mouse IgG1, K Isotype Control (left) or 0.06 ug of APC anti-rat CD25 (right). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of rat splenocytes with FITC anti-rat CD4 and 0.06 ug of APC Mouse IgG1, K Isotype Control (left) or 0.06 ug of APC anti-rat CD25 (right). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of rat splenocytes with FITC anti-rat CD4 and 0.06 ug of APC Mouse IgG1, K Isotype Control (left) or 0.06 ug of APC anti-rat CD25 (right). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of rat splenocytes with FITC anti-rat CD4 and 0.06 ug of APC Mouse IgG1, K Isotype Control (left) or 0.06 ug of APC anti-rat CD25 (right). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of rat splenocytes with FITC anti-rat CD4 and 0.06 ug of APC Mouse IgG1, K Isotype Control (left) or 0.06 ug of APC anti-rat CD25 (right). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/10/2018
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