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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C104666-50 50 µg $285 
LS-C104666-100 100 µg $325 
IL1A / IL-1 Alpha Antibody - Western Blot (reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL1A / IL-1 Alpha Antibody - Western Blot (non-reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL1A / IL-1 Alpha Antibody - Sandwich ELISA of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL1A / IL-1 Alpha Antibody - Western Blot (reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL1A / IL-1 Alpha Antibody - Western Blot (non-reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL1A / IL-1 Alpha Antibody - Sandwich ELISA of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IL1A / IL‑1 Alpha Antibody LS‑C104666

IL1A / IL‑1 Alpha Antibody LS‑C104666

Note: This antibody replaces LS-C7681, LS-C54103, LS-C42229
Antibody:
Rabbit Polyclonal to Mouse IL1A / IL-1 Alpha
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$285 
LS-C104666-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Mouse IL1A / IL-1 Alpha
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
IL-1 Alpha antibody LS-C104666 is an unconjugated rabbit polyclonal antibody to mouse IL-1 Alpha (IL1A ). Validated for ELISA and WB.
Target
Mouse IL1A / IL-1 Alpha
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • Applications tested for the base form of this product only
Immunogen
E.coli derived recombinant Murine IL-1alpha.
Specificity
Mouse IL-1alpha
Usage
Immunohistochemistry: This antibody stained colchicine injected mouse brain (including the hippocampal fissure) tissue. The primary antibody was incubated at 1 mg/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mIL-1alpha (0.05 ng/ml), a concentration of 0 3-0.05 ug/ml of this antibody is required. ELISA: To detect mIL-1alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-1alpha (LS-C104815) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1alpha. Western Blot: To detect mIL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL1A / IL-1 Alpha
P01583 NM_000575 NP_000566.3

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Requested From: United States
Date Requested: 12/5/2019