LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us

IL1A / IL-1 Alpha Antibody (Biotin) LS-C104815

Note: This antibody replaces LS-C7682, LS-C54104, LS-C42230
IL-1 Alpha antibody LS-C104815 is a biotin-conjugated rabbit polyclonal antibody to mouse IL-1 Alpha (IL1A). Validated for ELISA and WB.
Catalog
Size
Price
LS-C104815-25
25 µg
$285
LS-C104815-50
50 µg
$325
Description
IL-1 Alpha antibody LS-C104815 is a biotin-conjugated rabbit polyclonal antibody to mouse IL-1 Alpha (IL1A). Validated for ELISA and WB.
Target
Mouse IL1A / IL-1 Alpha
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available Unconjugated.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.25 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
E.coli derived recombinant Murine IL-1alpha.
Specificity
Mouse IL-1alpha
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mIL-1alpha by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1alpha. To detect mIL-1alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IL-1alpha (LS-C104666) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1alpha. Western Blot: To detect mIL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile PBS containing 0.1% BSA
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About IL1A / IL-1 Alpha
P01583 NM_000575 NP_000566.3

Popular IL1A / IL-1 Alpha Products

Anti-IL1 Alpha antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:400.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Western Blot (reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Western blot, ELISA
Immunohistochemistry Image
Species: Human
Applications: IHC, Western blot, ELISA
Western Blot (reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rat
Applications: Western blot, ELISA
IHC of paraffin-embedded Human lymph node tissue using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Publications (0)


Customer Reviews (0)


Images

Western blot

Biotinylated Anti-Murine IL-1a Western Blot Unreduced
Biotinylated Anti-Murine IL-1a Western Blot Unreduced

Western blot

Biotinylated Anti-Murine IL-1a Western Blot Reduced
Biotinylated Anti-Murine IL-1a Western Blot Reduced

Western blot

Western Blot (reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Biotinylated Anti-Murine IL-1a Sandwich ELISA
Biotinylated Anti-Murine IL-1a Sandwich ELISA

Western blot

Biotinylated Anti-Murine IL-1a Western Blot Unreduced
Biotinylated Anti-Murine IL-1a Western Blot Unreduced

Western blot

Biotinylated Anti-Murine IL-1a Western Blot Reduced
Biotinylated Anti-Murine IL-1a Western Blot Reduced

Western blot

Western Blot (reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Biotinylated Anti-Murine IL-1a Sandwich ELISA
Biotinylated Anti-Murine IL-1a Sandwich ELISA

Western blot

Biotinylated Anti-Murine IL-1a Western Blot Unreduced
Biotinylated Anti-Murine IL-1a Western Blot Unreduced

Western blot

Biotinylated Anti-Murine IL-1a Western Blot Reduced
Biotinylated Anti-Murine IL-1a Western Blot Reduced

Western blot

Western Blot (reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Biotinylated Anti-Murine IL-1a Sandwich ELISA
Biotinylated Anti-Murine IL-1a Sandwich ELISA

Western blot

Biotinylated Anti-Murine IL-1a Western Blot Unreduced
Biotinylated Anti-Murine IL-1a Western Blot Unreduced

Western blot

Biotinylated Anti-Murine IL-1a Western Blot Reduced
Biotinylated Anti-Murine IL-1a Western Blot Reduced

Western blot

Western Blot (reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Biotinylated Anti-Murine IL-1a Sandwich ELISA
Biotinylated Anti-Murine IL-1a Sandwich ELISA

Requested From: United States
Date Requested: 3/25/2019
Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2019 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy