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Catalog Number Size Price
LS-C104583-50 50 µg $356 
LS-C104583-100 100 µg $396 
IL1A / IL-1 Alpha Antibody - Western Blot (reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL1A / IL-1 Alpha Antibody - Western Blot (non-reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL1A / IL-1 Alpha Antibody - Sandwich ELISA of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL1A / IL-1 Alpha Antibody - Western Blot (reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL1A / IL-1 Alpha Antibody - Western Blot (non-reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL1A / IL-1 Alpha Antibody - Sandwich ELISA of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Polyclonal Goat anti‑Rat IL1A / IL‑1 Alpha Antibody (WB) LS‑C104583

Polyclonal Goat anti‑Rat IL1A / IL‑1 Alpha Antibody (WB) LS‑C104583

Note: This antibody replaces LS-C7660, LS-C7664, LS-C86135, LS-C36976, LS-C42292, LS-C54105
Antibody:
IL1A / IL-1 Alpha Goat anti-Rat Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$356
LS-C104583-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IL1A / IL-1 Alpha Goat anti-Rat Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
IL-1 Alpha antibody LS-C104583 is an unconjugated goat polyclonal antibody to rat IL-1 Alpha (IL1A). Validated for ELISA and WB.
Target
Rat IL1A / IL-1 Alpha
Synonyms
IL1A | Hematopoietin-1 | IL-1 alpha | IL1 | IL1-ALPHA | IL1 Alpha | Interleukin-1 alpha | IL-1A | IL1F1 | Pro-interleukin-1-alpha | Preinterleukin 1 alpha | Interleukin 1, alpha
Host
Goat
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Rat IL-1alpha.
Specificity
Rat IL-1alpha
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
Usage
Immunohistochemistry: This antibody stained colchicine injected rat brain (including the ventricles and the CA3 region of the hippocampus) tissue. The primary antibody was incubated at 1 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat IL-1alpha (50 pg/ml), a concentration of 0.011-0.017 ug/ml of this antibody is required. ELISA: To detect Rat IL-1alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-1alpha (LS-C104576) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-1alpha. Western Blot: To detect Rat IL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute in sterile water to 0.1-1 mg/ml.
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL1A / IL-1 Alpha
P01583 NM_000575 NP_000566.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/14/2024