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Catalog Number Size Price
LS-C745233-25 25 µl (1 mg/ml) $275 
IL1A / IL-1 Alpha Antibody - Western Blot of rabbit Anti-Human IL-1 alpha Antibody. Lane 1: Opal Prestained Molecular Weight Ladder Lane 2: IL-1 alpha 50ng. Lane 3: MEF WC lysate 10ng. Lane 4: MEF LPS stimulated 10ng. Blocking: BlockOut Buffer for 30min at RT. Primary Antibody: Anti-IL-1a 1µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP at 1:70, 000) for 30min at RT.

Polyclonal Rabbit anti‑Human IL1A / IL‑1 Alpha Antibody (IHC, WB) LS‑C745233

Polyclonal Rabbit anti‑Human IL1A / IL‑1 Alpha Antibody (IHC, WB) LS‑C745233

Antibody:
IL1A / IL-1 Alpha Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, Flo, ELISA, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745233-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
IL1A / IL-1 Alpha Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, Flo, ELISA, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IL-1 Alpha antibody LS-C745233 is an unconjugated rabbit polyclonal antibody to human IL-1 Alpha (IL1A). Validated for ELISA, Flow, IHC, Neut and WB.
Target
Human IL1A / IL-1 Alpha
Synonyms
IL1A | Hematopoietin-1 | IL-1 alpha | IL1 | IL1-ALPHA | IL1 Alpha | Interleukin-1 alpha | IL-1A | IL1F1 | Pro-interleukin-1-alpha | Preinterleukin 1 alpha | Interleukin 1, alpha
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
DEAE fractionation
Modifications
Unmodified
Immunogen
The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1a produced in E.coli.  The MW of the recombinant IL-1a was 17,000.  This is the cleavage site generated by the IL-1b converting enzyme (ICE, capase-1).
Specificity
This antibody is primarily directed against the 17,000 MW human IL-1a and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1a in the same formats using similar dilutions. The antiserum does not recognize human IL-1b or Mouse or Rabbit IL-1a. In ELISA formats and other immunoreactive assays, this antibody will recognize both the mature 17,000 MW IL-1a as well as the 31,000 MW IL-1a precursor in either non-denatured (native) or denatured samples. Unlike the IL-1b precursor, the native precursor of IL-1a is recognized by the antibody produced to the 17,000 MW form. The 31,000 precursor of IL-1a is biologically active and is found primarily intracellularly. The precursor of IL-1a, unlike that of IL-1b, is biologically active when applied to cells and is thought to have a role as a functional molecule intracellularly and can be found constitutively expressed in various cell. This antibody is also useful for neutralization of human and primate IL-1a activity in bioassays. It does not neutralize the biological activity IL-1b. It does not neutralize the biological activity of mouse, rat or rabbit IL-1a. It will neutralize primate IL-a. For neutralization, it is recommended to incubate the sample with a 1:100 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F( c) domain of the rabbit IgG molecule may interact with cells non-specifically.
Applications
  • IHC (1:100 - 1:200)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry
  • ELISA (1:1000 - 1:5000)
  • Neutralization
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL1A / IL-1 Alpha
P01583 NM_000575 NP_000566.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021