Human THBS1 / Thrombospondin-1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein G purified

Azide-free

Purified human TSP.

Designed for sensitive detection of TSP by Western Blot. It shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Species cross-reactivity: human.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot

Immunohistochemistry: LS-B2156 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2156 was determined to be 5 ug/ml. Western Blot: Reduced and non-reduced. 0.5-1.0 ug/ml for 2 hours at RT. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS, pH 7.4

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P07996 NCBI: NM_003246 NP_003237.2