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Catalog Number Size Price
LS-B4155-50 50 µg (0.5 mg/ml) $485 
THBS1 / Thrombospondin-1 Antibody - Anti-THBS1 / Thrombospondin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
THBS1 / Thrombospondin-1 Antibody - Anti-THBS1 / Thrombospondin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
THBS1 / Thrombospondin-1 Antibody - THBS1 / Thrombospondin-1 antibody immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/Plasma Membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
THBS1 / Thrombospondin-1 Antibody - THBS1 / Thrombospondin-1 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
THBS1 / Thrombospondin-1 Antibody - THBS1 / Thrombospondin-1 antibody (1µg/ml) staining of Mouse Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
THBS1 / Thrombospondin-1 Antibody - THBS1 / Thrombospondin-1 antibody flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
THBS1 / Thrombospondin-1 Antibody - Anti-THBS1 / Thrombospondin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
THBS1 / Thrombospondin-1 Antibody - Anti-THBS1 / Thrombospondin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
THBS1 / Thrombospondin-1 Antibody - THBS1 / Thrombospondin-1 antibody immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/Plasma Membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
THBS1 / Thrombospondin-1 Antibody - THBS1 / Thrombospondin-1 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
THBS1 / Thrombospondin-1 Antibody - THBS1 / Thrombospondin-1 antibody (1µg/ml) staining of Mouse Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
THBS1 / Thrombospondin-1 Antibody - THBS1 / Thrombospondin-1 antibody flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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IHC‑plus™ Polyclonal Goat anti‑Human THBS1 / Thrombospondin‑1 Antibody (aa19‑32, IHC, IF, WB) LS‑B4155

IHC‑plus™ Polyclonal Goat anti‑Human THBS1 / Thrombospondin‑1 Antibody (aa19‑32, IHC, IF, WB) LS‑B4155

Note: This antibody replaces LS-C108341
Antibody:
THBS1 / Thrombospondin-1 Goat anti-Human Polyclonal (aa19-32) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Bovine, Horse, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B4155-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
THBS1 / Thrombospondin-1 Goat anti-Human Polyclonal (aa19-32) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Bovine, Horse, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
Thrombospondin-1 antibody LS-B4155 is an unconjugated goat polyclonal antibody to Thrombospondin-1 (THBS1) (aa19-32) from human. It is reactive with human, mouse, bovine and other species. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human THBS1 / Thrombospondin-1
Synonyms
THBS1 | Thrombospondin-1 | Thrombospondin-1p180 | TSP | TSP1 | THBS-1 | THBS | Thrombospondin 1 | TSP-1
Host
Goat
Reactivity
Human, Monkey, Mouse, Bovine, Horse, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence NRIPESGGDNSVFD-C, from the N-Terminus of protein sequence according to NP_003237.2.
Epitope
aa19-32
Specificity
Human THBS1 / Thrombospondin.
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B4155 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4155 was determined to be 3.75 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About THBS1 / Thrombospondin-1
P07996 NM_003246 NP_003237.2

Validation

THBS1 / Thrombospondin-1 Antibody - Anti-THBS1 / Thrombospondin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-THBS1 / Thrombospondin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-THBS1 / Thrombospondin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

THBS1 / Thrombospondin-1 Antibody - Anti-THBS1 / Thrombospondin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-THBS1 / Thrombospondin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-THBS1 / Thrombospondin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

LSBio Ratings

IHC-plus™ THBS1 / Thrombospondin-1 Antibody (aa19-32) for IHC, IF/Immunofluorescence, WB/Western LS-B4155 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability. Schips TG, Vanhoutte D, Vo A, Correll RN, Brody MJ, Khalil H, Karch J, Tjondrokoesoemo A, Sargent MA, Maillet M, Ross RS, Molkentin JD. Nature communications. 2019 January;10:76.

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Requested From: United States
Date Requested: 7/22/2024