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Catalog Number Size Price
LS-B2570-50 50 µl (1 mg/ml) $485 
THBS1 / Thrombospondin-1 Antibody - Anti-Thrombospondin antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

IHC‑plus™ Monoclonal Mouse anti‑Human THBS1 / Thrombospondin‑1 Antibody (clone A6.1, Azide‑free, IHC, IF, WB) LS‑B2570

IHC‑plus™ Monoclonal Mouse anti‑Human THBS1 / Thrombospondin‑1 Antibody (clone A6.1, Azide‑free, IHC, IF, WB) LS‑B2570

Note: This antibody replaces LS-C26351, LS-C87486
Antibody:
THBS1 / Thrombospondin-1 Mouse anti-Human Monoclonal (Azide-free) (A6.1) Antibody
Application:
IHC, IHC-P, IF, WB, IP, Flo
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep
Format:
Unconjugated, Azide-free
Other formats:
Price
Catalog Number
$485
LS-B2570-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
THBS1 / Thrombospondin-1 Mouse anti-Human Monoclonal (Azide-free) (A6.1) Antibody
Application:
IHC, IHC-P, IF, WB, IP, Flo
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep
Format:
Unconjugated, Azide-free
Other formats:

Specifications

Description
Thrombospondin-1 antibody LS-B2570 is an unconjugated mouse monoclonal antibody to Thrombospondin-1 (THBS1) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human THBS1 / Thrombospondin-1
Synonyms
THBS1 | Thrombospondin-1 | Thrombospondin-1p180 | TSP | TSP1 | THBS-1 | THBS | Thrombospondin 1 | TSP-1
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
A6.1
Conjugations
Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Azide-free
Immunogen
Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP
Specificity
Recognizes Thrombospondin. Shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild cross-reaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e. g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i. e. at low concentration of Ca2+. Species cross-reactivity: Human, bovine, porcine, equine, canine, sheep, mouse and rat.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2570 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2570 was determined to be 10 ug/ml. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About THBS1 / Thrombospondin-1
P07996 NM_003246 NP_003237.2

Validation

THBS1 / Thrombospondin-1 Antibody - Anti-Thrombospondin antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-Thrombospondin antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-Thrombospondin antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

LSBio Ratings

IHC-plus™ THBS1 / Thrombospondin-1 Antibody (clone A6.1, Azide-free) for IHC, IF/Immunofluorescence, WB/Western, IP, Flow LS-B2570 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/21/2024