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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B114-100 100 µg (1 mg/ml) $425 
NR4A1 / NUR77 Antibody - Anti-NUR77 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
NR4A1 / NUR77 Antibody - Western blot of Nak-1 in 15 ugs of NIH3T3 cell lysate using antibody at 1:500 dilution.
NR4A1 / NUR77 Antibody - Anti-NUR77 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
NR4A1 / NUR77 Antibody - Western blot of Nak-1 in 15 ugs of NIH3T3 cell lysate using antibody at 1:500 dilution.
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IHCPlus™ Polyclonal NR4A1 / NUR77 Antibody (aa251‑266, IHC, IF, WB) LS‑B114

IHCPlus™ Polyclonal NR4A1 / NUR77 Antibody (aa251‑266, IHC, IF, WB) LS‑B114

Note: This antibody replaces LS-C762, LS-C73134
Antibody:
Rabbit Polyclonal to Human NR4A1 / NUR77
Application:
IHC, IHC-P, ICC, IF, WB, IP, ChrIP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B114-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human NR4A1 / NUR77
Application:
IHC, IHC-P, ICC, IF, WB, IP, ChrIP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NUR77 antibody LS-B114 is an unconjugated rabbit polyclonal antibody to NUR77 (NR4A1) (aa251-266) from human, mouse and rat. Validated for ChrIP, ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human NR4A1 / NUR77
Synonyms
NR4A1 | GFRP1 | Early response protein NAK1 | HMR | NGFIB | Orphan nuclear receptor TR3 | Orphan receptor tr3 | Steroid receptor TR3 | TR3 | NAK1 | NGFI-B | NP10 | Receptor NGFIB | Testicular receptor 3 | TR3 orphan receptor | N10 | NAK-1 | NUR77 | Orphan nuclear receptor HMR
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (20 - 40 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:500)
  • Chromatin Immunoprecipitation (1:10 - 1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human NR4A1 / NUR77.
Epitope
aa251-266
Specificity
A synthetic peptide corresponding to aa 251-266 of human Nak1.
Usage
Immunohistochemistry: LS-B114 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B114 was determined to be 20-40 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NR4A1 / NUR77
P22736 NM_002135 NP_002126.2

Validation

NR4A1 / NUR77 Antibody - Anti-NUR77 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Anti-NUR77 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Anti-NUR77 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

LSBio Ratings

IHC-plus™ NR4A1 / NUR77 Antibody (aa251-266) for IHC, ICC, IF/Immunofluorescence, WB/Western, IP LS-B114 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)

Publications (2)

Nuclear receptor NR4A1 is a tumor suppressor down-regulated in triple-negative breast cancer. Wu H #, Bi J #, Peng Y #, Huo L #, Yu X, Yang Z, Zhou Y, Qin L, Xu Y, Liao L, Xie Y, Conneely OM, Jonkers J, Xu J,. Oncotarget. 2017 8:54364-54377. (ICC; Human)

Nur77 gene expression levels were involved in different ACTH-secretion autonomy between Cushing's disease and subclinical Cushing's disease. Tabuchi Y, Kitamura T, Fukuhara A, Mukai K, Onodera T, Miyata Y, Hamasaki T, Oshino S, Saitoh Y, Morii E, Otsuki M, Shimomura I. Endocrine Journal. 2016 June;63:545-554.

Customer Reviews (0)


Requested From: United States
Date Requested: 4/9/2020