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IHCPlus™ NR4A1 / NUR77 Antibody aa401‑414 Polyclonal IHC,Peptide‑ELISA,WB LS‑B2456

Catalog Number Size Price
LS-B2456-50 50 µg (0.5 mg/ml) $425 
Anti-NUR77 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Antibody (2 ug/ml) staining of Human Thyroid lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-NUR77 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Antibody (2 ug/ml) staining of Human Thyroid lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ NR4A1 / NUR77 Antibody aa401‑414 Polyclonal IHC,Peptide‑ELISA,WB LS‑B2456

Note: This antibody replaces LS-C55192, LS-C73800
Antibody:
Goat Polyclonal to Human NR4A1 / NUR77
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Dog, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B2456-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human NR4A1 / NUR77
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Dog, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
NUR77 antibody LS-B2456 is an unconjugated goat polyclonal antibody to NUR77 (NR4A1 ) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NR4A1 / NUR77
Host
Goat
Reactivity
Human, Mouse, Rat, Dog, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-GRLPSKPKQPPDAS, from the internal region of the protein sequence according to NP_002126.2NP_775180.1.
Blocking Peptide
LS-E26163 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2456. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa401-414
Specificity
Human NR4A1 / NUR77. This antibody is expected to recognise isoform a (NP_002126.2 and NP_775180.1) only.
Usage
Immunohistochemistry: LS-B2456 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2456 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NR4A1 / NUR77
P22736 NM_002135 NP_002126.2

Validation

Anti-NUR77 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-NUR77 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NUR77 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 9/17/2019